Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2021-08-20 , DOI: 10.1007/s00216-021-03620-0 Donghao Liu 1, 2 , Ping Yang 1 , Fangqi Wang 1 , Cong Wang 3 , Li Chen 1 , Sijing Ye 1 , Pierre Dramou 1, 2 , Jianhua Chen 3 , Hua He 1, 2, 4
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Nanozymes were the novel research field to replace natural enzymes because of stability and low cost. However, the research on nanozymes was mainly focused on peroxidase, and there was little research about nanozymes with oxidase-like activity, especially mimic oxidase of small molecules related to human physiology. High levels of uric acid (UA) in the body can cause hyperuricemia and gout. And natural uricase cured this disease because it could oxidize UA. The oxidase-like activity of mixed valence state metal organic frameworks with cerium (MVSM) had been studied, but MVSM was found to have uricase-like activity in this article. The catalytic process of UA with MVSM was studied by a variety of analytical methods, which was similar to the natural uricase except for further oxidation of H2O2. The catalytic activity constants of MVSM were acquired by the Michaelis-Menten equation. MVSM had a better ability to catalyze UA in in vivo and in vitro experiments. An enzyme-free analysis–based mimic uricase for UA was established. All the experimental results proved that MVSM had a good prospect to replace the natural uricase.
Graphical abstract
中文翻译：
模拟尿酸酶性能研究及其在无酶分析中的应用
纳米酶因其稳定性和成本低而成为替代天然酶的新研究领域。然而，纳米酶的研究主要集中在过氧化物酶上，对具有类似氧化酶活性的纳米酶，尤其是模拟与人体生理相关的小分子氧化酶的研究较少。体内高水平的尿酸 (UA) 会导致高尿酸血症和痛风。天然尿酸酶治愈了这种疾病，因为它可以氧化 UA。已经研究了混合价态金属有机骨架与铈 (MVSM) 的氧化酶样活性，但在本文中发现 MVSM 具有类似尿酸酶的活性。通过多种分析方法研究了UA与MVSM的催化过程，除进一步氧化H 2 O 2外，与天然尿酸酶相似. MVSM 的催化活性常数通过 Michaelis-Menten 方程获得。MVSM在体内和体外实验中具有更好的催化UA的能力。建立了一种基于无酶分析的 UA 模拟尿酸酶。所有的实验结果证明MVSM具有很好的替代天然尿酸酶的前景。
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