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Study on performance of mimic uricase and its application in enzyme-free analysis

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Abstract

Nanozymes were the novel research field to replace natural enzymes because of stability and low cost. However, the research on nanozymes was mainly focused on peroxidase, and there was little research about nanozymes with oxidase-like activity, especially mimic oxidase of small molecules related to human physiology. High levels of uric acid (UA) in the body can cause hyperuricemia and gout. And natural uricase cured this disease because it could oxidize UA. The oxidase-like activity of mixed valence state metal organic frameworks with cerium (MVSM) had been studied, but MVSM was found to have uricase-like activity in this article. The catalytic process of UA with MVSM was studied by a variety of analytical methods, which was similar to the natural uricase except for further oxidation of H2O2. The catalytic activity constants of MVSM were acquired by the Michaelis-Menten equation. MVSM had a better ability to catalyze UA in in vivo and in vitro experiments. An enzyme-free analysis–based mimic uricase for UA was established. All the experimental results proved that MVSM had a good prospect to replace the natural uricase.

Graphical abstract

A nanomaterial, mixed valence state Ce-MOF (MVSM), with uricase-like activity has been found in vivo and in vitro. This material has potential to be a fluorescent analysis for detecting uric acid without uricase.

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Funding

This work was financially supported by the Natural Science Foundation Projects of China (No. 81950410634), the Bureau of the International Cooperation of the NSFC and the Foreign Experts Department of the Ministry of Science and Technology of the People’s Republic of China through a Grant for Foreign Youth Project (No. QNJ20200010003), the Independent Innovation Fund Project of Agricultural Science and Technology of Jiangsu Province in 2017 (NoCX (17) 1003), and the Innovation and Entrepreneurship Training Program for Undergraduate (No. 202010316209).

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Authors and Affiliations

  1. Department of Analytical Chemistry, China Pharmaceutical University, 639 Longmian Avenue, Nanjing, 211198, Jiangsu, China

    Donghao Liu, Ping Yang, Fangqi Wang, Li Chen, Sijing Ye, Pierre Dramou & Hua He

  2. Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China

    Donghao Liu, Pierre Dramou & Hua He

  3. School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, Jiangsu, China

    Cong Wang &  Jianhua Chen

  4. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China

    Hua He

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  1. Donghao Liu
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  2. Ping Yang
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  4. Cong Wang
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  9. Hua He
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Correspondence to Jianhua Chen or Hua He.

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Urine sample was supported by healthy people who had signed a written informed consent to participate in this research. Ethics approval of animal tests and using human urine samples was obtained from the Ethics Committee of China Pharmaceutical University School. The study was performed in accordance with the ethical standards.

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Liu, D., Yang, P., Wang, F. et al. Study on performance of mimic uricase and its application in enzyme-free analysis. Anal Bioanal Chem 413, 6571–6580 (2021). https://doi.org/10.1007/s00216-021-03620-0

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  • DOI: https://doi.org/10.1007/s00216-021-03620-0

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