Three-dimensional flowerlike Au@(MoS₂/GO/o-MWNTs) nanohybrids (abbreviated as AMGMs) were synthesized and then introduced into an electrochemiluminescence (ECL) system as a new co-reaction accelerator for the ultrasensitive prostate-specific antigen (PSA). The AMGMs not only served as a substrate with good conductivity and a large specific surface area for loading abundant primary antibodies but also acted as an effective co-reaction accelerator; the co-reaction accelerator could interact with a co-reactant rather than the luminophore to boost the generation of free radical intermediates, thereby producing abundant excited states of luminophores to amplify the ECL signal response. Additionally, an anticipated signal amplification strategy based on the hybridization chain reaction (HCR) was developed by gathering a large amount of a DNA initiator on gold nanoparticles. These gathered DNA initiators could generate multiple DNA concatemers and attach more signal molecules, which resulted in outstanding exponential signal amplification. Consequently, the ECL immunosensor demonstrated high sensitivity, with a linear range from 0.1 pg mL⁻¹ to 50 ng mL⁻¹ and a detection limit of 0.028 pg mL⁻¹. In addition, the immunosensor displayed excellent stability and selectivity. It was evaluated by analyzing human serum sample. The recovery obtained was 98.80–112.00% and the RSD was 1.73–3.12%, indicating the immunosensor could be applied to the simultaneous detection of PSA in human serum samples.

三维花状Au@(MoS ₂/GO/o-MWNTs）纳米杂化物（缩写为 AMGMs）被合成，然后被引入电化学发光（ECL）系统作为超敏感前列腺特异性抗原（PSA）的新型共反应加速器。AMGMs 不仅可以作为一种具有良好导电性和大比表面积的底物来装载丰富的一抗，而且还可以作为一种有效的共反应加速剂；共反应加速器可以与共反应物而不是发光体相互作用，以促进自由基中间体的产生，从而产生丰富的发光体激发态以放大 ECL 信号响应。此外，通过在金纳米粒子上收集大量 DNA 引发剂，开发了基于杂交链反应 (HCR) 的预期信号放大策略。这些聚集的 DNA 起始子可以产生多个 DNA 串联体并附着更多的信号分子，从而导致出色的指数信号放大。因此，ECL 免疫传感器表现出高灵敏度，线性范围从 0.1 pg mL^-1至 50 ng mL ^-1和 0.028 pg mL ^-1的检测限。此外，免疫传感器显示出优异的稳定性和选择性。它是通过分析人血清样品来评估的。获得的回收率为 98.80-112.00%，RSD 为 1.73-3.12%，表明免疫传感器可用于同时检测人血清样品中的 PSA。