Amethod for quantitative determination of aloenin by microcolumn reversed-phase HPLC with UV detection (306 nm) using a KAH-6-80-4 column (2 × 80 mm, Separon-C18, 7 μm), mobile phase MeCN–aqueous HOAc (1%) (25:75), elution rate 100 μL/min, and eluent volume 2000 μL was developed. Validation showed that the method was characterized satisfactory metrological parameters. The limit of quantitation for aloenin was 115 mg/mL. The accuracy at an aloenin content level within 80 – 120% was 96.54 – 103.28%. The method was used for analysis of Aloe arborescens Mill. leaves, ex tempore obtained juice, and some medicines (Aloe liquid extract for subcutaneous injection, Aloe juice). Statistical processing indicated that the relative error of average results for the aloenin assay did not exceed 5% with a confidence probability of 95%.

使用 KAH-6-80-4 色谱柱（2 × 80 mm，Separon-C18，7 μm），通过微柱反相 HPLC 和 UV 检测（306 nm）定量测定芦荟素，流动相 MeCN-HOAc 水溶液（ 1%) (25:75)，洗脱速率为 100 μL/min，洗脱液体积为 2000 μL。验证表明该方法具有令人满意的计量参数。芦荟素的定量限为 115 mg/mL。芦荟素含量在 80-120% 范围内的准确度为 96.54-103.28%。该方法用于木香芦荟的分析。叶子，临时得到的汁液和一些药物（芦荟皮下注射液提取物、芦荟汁）。统计处理表明芦荟素测定平均结果的相对误差不超过5%，置信概率为95%。