Amethod for quantitative determination of aloenin by microcolumn reversed-phase HPLC with UV detection (306 nm) using a KAH-6-80-4 column (2 × 80 mm, Separon-C18, 7 μm), mobile phase MeCN–aqueous HOAc (1%) (25:75), elution rate 100 μL/min, and eluent volume 2000 μL was developed. Validation showed that the method was characterized satisfactory metrological parameters. The limit of quantitation for aloenin was 115 mg/mL. The accuracy at an aloenin content level within 80 – 120% was 96.54 – 103.28%. The method was used for analysis of Aloe arborescens Mill. leaves, ex tempore obtained juice, and some medicines (Aloe liquid extract for subcutaneous injection, Aloe juice). Statistical processing indicated that the relative error of average results for the aloenin assay did not exceed 5% with a confidence probability of 95%.
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References
I. N. Zilfikarov, D. N. Olennikov, T. A. Ibragimov, et al., Current Aspects of Pharmacognostic and Biochemical Studies of Succulent Raw Material of Aloe arborescens and Callisia fragrans [in Russian], Izdatel’ Markhotin P. Yu., MO, Shchelkovo (2013), pp. 12 – 30.
V. K. Lavrenov and G. V. Lavrenov, Complete Encyclopedia of Medicinal Plants [in Russian], in 2 vols., Olma-Press, Moscow (1999), Vol. 2, 267 pp.
A. N. B. Singab, H. M. El-Hefnawy, A. Esmat, et al., Phytother. Res., 29, 1858 – 1867 (2015).
B. Salehi, S. Albayrak, H. Antolak, et al., Int. J. Mol. Sci., 19(9), 2843 (2018).
M. Yamamoto, T. Masui, K. Sugiyama, et al., Agric. Biol. Chem., 55(6), 1627 – 1629 (1991).
I. E. Cock, “The genus Aloe: Phytochemistry and therapeutic uses including treatments for gastrointestinal conditions and chronic inflammation,” in: Novel Natural Products: Therapeutic Effects in Pain, Arthritis and Gastro-intestinal Diseases, K. D. Rainsford, et al. (eds.), Springer Basel (2015), pp. 179 – 235.
H. Beppu, T. Koike, K. Shimpo, et al., J. Ethnopharmacol., 83, 37 – 45 (2003).
S. Kameyama and M. Shinpo, JP Pat. S54 – 151113, Nov. 28, 1979; Chem. Abstr., 93, 10375y (1980).
European Medicines Agency. Assessment report on Aloe barbadensis Mill. and on Aloe (various species, mainly Aloe ferox Mill. and its hybrids), folii succus siccatus, 22 November 2016. EMA/HMPC/759585/2015: URL: https://www.ema.europa.eu/en/documents/herbal-report/final-assessment-report-aloe-barbadensis-mill-aloe-various-species-mainly-aloe-ferox-mill-itsen.pdf (accessed on Feb. 1, 2021).
A. I. Pavlov and A. A. Pavlova, Doktor Ru, No. 3(158), 47 – 52 (2019).
M. E. Clementi, G. Tringali, D. Triggiani, and B. Giardina, Nat. Prod. Commun., 10, 1993 – 1995 (2015).
S. E. Kudritskaya, G. M. Fishman, L. M. Zagorodskaya, and D. M. Chikovani, Khim. Prir. Soedin., No. 4, 573 (1985).
D. K. Patel, K. Patel, and V. Tahilyani, Asian Pac. J. Trop. Biomed., 2(10), 835 – 838 (2012).
D. N. Olennikov, I. N. Zilfikarov, and T. A. Ibragimova, Khim. Rastit. Syr’ya, No. 3, 77 – 82 (2010).
S. W. Kwon, J. H. Park, Y. I. Park, et al., “Chemical components of aloe and its analysis,” in: New Perspectives on Aloe, Y. I. Park and S. K. Lee (eds.), Springer, Boston, MA (2006), pp. 19 – 62.
H. Beppu, K. Kawai, K. Shimpo, et al., Biochem. Syst. Ecol., 32(9), 783 – 795 (2004).
State Drug Registry, 2020; http://grls.rosminzdrav.ru/grls.aspx.
S. N. Glushchenko, A. A. Shmygareva, and A. N. San’kov, Aspir. Vestn. Povolzh’ya, No. 1 – 2, 126 – 130 (2020).
State Pharmacopoeia of the Russian Federation, XIVth Ed., Vol. IV, Moscow (2018).
European Pharmacopoeia, 9th Ed., EDQM, European Pharmacopoeia, Council of Europe, B. P. 907, F-67029, Strasbourg, France (2017).
E. V. Sergunova and A. A. Sorokina, Farmatsiya, 68(7), 21 – 25 (2019).
D. N. Olennikov, A. V. Rokhin, and I. N. Zilfikarov, Chem. Nat. Compd., 44(6), 715 – 718 (2008).
D. N. Olennikov, I. N. Zilfikarov, and T. A. Penzina, Khim-farm. Zh., 47(9), 42 – 45 (2013).
State Pharmacopoeia of the Russian Federation, XIVth Ed., Vol. 1, Moscow (2018).
Y. Guttermana and E. Chauser-Volfson, Int. J. Food Sci. Technol., 41(6), 662 – 666 (2006).
A. Kodym, A. Marcinkowski, and H. Kukula, Acta Pol. Pharm., 60(1), 31 – 39 (2003).
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 55, No. 5, pp. 13 – 18, May, 2021.
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Kurkin, V.A., Ryazanova, T.K., Shmygareva, A.A. et al. HPLC Determination of Aloenin in Leaves and Preparations of Aloe arborescens Mill.. Pharm Chem J 55, 454–459 (2021). https://doi.org/10.1007/s11094-021-02442-9
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DOI: https://doi.org/10.1007/s11094-021-02442-9