Monatshefte für Chemie - Chemical Monthly ( IF 1.7 ) Pub Date : 2021-08-16 , DOI: 10.1007/s00706-021-02809-4 Anastasia V. Kravchenko 1 , Ekaterina A. Kolobova 1, 2 , Liudmila A. Kartsova 1
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Nowadays, there are plenty of approaches to determine steroids hormones by capillary electrophoresis, however, the development of new ones is still actual. In the present study, the possibilities of 3-methyl-1-β-cyclodextrinimidazole tosylate as pseudo stationary phase are considered. Due to the formation of positively charged complexes between steroids with a derivative based on β-cyclodextrin and imidazolium cation, the following results were achieved: first, it was established that adding of this modifier at concentration 0.70 mM at pH 3.2 allowed to separate the five most important corticosteroid hormones (cortisone, hydrocortisone, 11-deoxycortisol, corticosterone, 21-hydroxyprogesterone). Second, the presence of a modifier in the sample permitted to carry out electrokinetic injection of samples and preconcentration of analytes under field-amplified sample injection mode. Obtained values of limits of detection (5–15 ng/cm3) allowed to analyze plasma samples after liquid–liquid extraction by chloroform.
Graphic abstract
中文翻译：
3-甲基-1-β-环糊精咪唑甲苯磺酸盐在毛细管电泳分离和皮质类固醇预浓缩中的应用
目前，通过毛细管电泳测定类固醇激素的方法很多，但新的方法仍在开发中。在本研究中，考虑了 3-methyl-1-β-cyclodextrinimidazole tosylate 作为假固定相的可能性。由于类固醇与基于 β-环糊精和咪唑鎓阳离子的衍生物之间形成带正电荷的复合物，因此获得了以下结果：首先，确定在 pH 3.2 下以 0.70 mM 的浓度添加该改性剂允许将五种最重要的皮质类固醇激素（可的松、氢化可的松、11-脱氧皮质醇、皮质酮、21-羟基孕酮）。第二，样品中改性剂的存在允许在场放大样品注射模式下进行样品的电动注射和分析物的预浓缩。获得的检测限值 (5–15 ng/cm3 ) 允许分析氯仿液液萃取后的血浆样品。
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