Abstract
Nowadays, there are plenty of approaches to determine steroids hormones by capillary electrophoresis, however, the development of new ones is still actual. In the present study, the possibilities of 3-methyl-1-β-cyclodextrinimidazole tosylate as pseudo stationary phase are considered. Due to the formation of positively charged complexes between steroids with a derivative based on β-cyclodextrin and imidazolium cation, the following results were achieved: first, it was established that adding of this modifier at concentration 0.70 mM at pH 3.2 allowed to separate the five most important corticosteroid hormones (cortisone, hydrocortisone, 11-deoxycortisol, corticosterone, 21-hydroxyprogesterone). Second, the presence of a modifier in the sample permitted to carry out electrokinetic injection of samples and preconcentration of analytes under field-amplified sample injection mode. Obtained values of limits of detection (5–15 ng/cm3) allowed to analyze plasma samples after liquid–liquid extraction by chloroform.
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References
Holst JP, Soldin OP, Guo T, Soldin SJ (2004) Clin Lab Med 24:105
Britz-McKibbin P, Ichihashi T, Tsubota K, Chen DDY, Terabe S (2003) J Chromatogr A 1013:65
Katayama M, Matsuda Y, Shimokawa K, Kaneko S (2003) Biomed Chromatogr 17:263
Baczek T, Oledzka I, Konieczna L, Kowalski P, Plenis A (2011) Sci World J 202:268120
Wu CH, Chen TH, Huang KP, Wang GR, Liu CY (2007) Electrophoresis 20:3691
Wu CH, Chen TH, Wang GR, Huang KP, Liu CY (2009) J Liq Chromatogr Relat Technol 32:833
Pomponio R, Gotti R, Fiori J, Cavrini V (2005) J Chromatogr A 1081:24
Flor S, Lucangioli S, Contin M, Tripodi V (2010) Electrophoresis 31:3305
Lokajová J, Railila A, King AW, Wiedmer SK (2013) J Chromatogr A 1308:144
Bessonova EA, Kartsova LA, Gallyamova VF (2016) J Anal Chem 71:696
Dzema D, Kartsova L, Emelianov G (2017) J Sep Sci 40:3335
Sirén H, Seppänen-Laakso T, Orešič M (2008) J Chromatogr B 871:375
Liu FK (2008) J Chromatogr A 1215:194
Wang CC, Cheng SF, Cheng HL, Chen YL (2013) Anal Bioanal Chem 405:1969
Wang CC, Chen JL, Chen YL, Cheng HL, Wu SM (2012) Anal Chim Acta 744:99
Kravchenko A, Kolobova E, Kartsova L (2020) Sep Sci Plus 3:102
Kolobova E, Kartsova L, Kravchenko A, Bessonova E (2018) Talanta 188:183
Brady B, Lynam N, O’Sullivan T, Ahern C, Darcy R (2004) Org Synth Coll 10:686
Muderawan IW, Ong TT, Tang W, Young DJ, Ching CB, Ng SC (2005) Tetrahedron Lett 46:1747
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This work was supported by Russian Science Foundation (Grant Number 19-13-00370).
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Kravchenko, A.V., Kolobova, E.A. & Kartsova, L.A. Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis. Monatsh Chem 152, 1067–1074 (2021). https://doi.org/10.1007/s00706-021-02809-4
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DOI: https://doi.org/10.1007/s00706-021-02809-4