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Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis

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Abstract

Nowadays, there are plenty of approaches to determine steroids hormones by capillary electrophoresis, however, the development of new ones is still actual. In the present study, the possibilities of 3-methyl-1-β-cyclodextrinimidazole tosylate as pseudo stationary phase are considered. Due to the formation of positively charged complexes between steroids with a derivative based on β-cyclodextrin and imidazolium cation, the following results were achieved: first, it was established that adding of this modifier at concentration 0.70 mM at pH 3.2 allowed to separate the five most important corticosteroid hormones (cortisone, hydrocortisone, 11-deoxycortisol, corticosterone, 21-hydroxyprogesterone). Second, the presence of a modifier in the sample permitted to carry out electrokinetic injection of samples and preconcentration of analytes under field-amplified sample injection mode. Obtained values of limits of detection (5–15 ng/cm3) allowed to analyze plasma samples after liquid–liquid extraction by chloroform.

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Acknowledgements

This work was supported by Russian Science Foundation (Grant Number 19-13-00370).

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Correspondence to Anastasia V. Kravchenko.

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Kravchenko, A.V., Kolobova, E.A. & Kartsova, L.A. Usage of 3-methyl-1-β-cyclodextrinimidazole tosylate for electrophoretic separation and preconcentration of corticosteroids by capillary electrophoresis. Monatsh Chem 152, 1067–1074 (2021). https://doi.org/10.1007/s00706-021-02809-4

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  • DOI: https://doi.org/10.1007/s00706-021-02809-4

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