Despite being widely available, Saccharomyces cerevisiae has not been widely explored for direct extraction of chitosan biopolymer for antimicrobial applications. In our study, S. cerevisiae from Baker’s yeast and Aspergillus niger from moldy onion extracts are studied as alternative sources of chitosan; and S cerevisiae chitosan tested for antimicrobial efficacy. The properties of S. cerevisiae chitosan are compared with moldy onion chitosan and shrimp chitosan extracted from shrimp shells. Chitosan extracted from S. cerevisiae is tested for antimicrobial efficacy against Staphylococcus Aureus. The maximum yields of fungal chitosan are 20.85 ± 0.35 mg/g dry S. cerevisiae biomass at 4th day using a culture broth containing sodium acetate, and 16.15 ± 0.95 mg/g dry A. niger biomass at 12th day. The degree of deacetylation (DD%) of the extracted fungal chitosan samples from S. cerevisiae and A. niger is found to be 63.4%, and 61.2% respectively, using Fourier Transform Infrared Spectroscopy. At a concentration of 2 g/L, S. cerevisiae chitosan shows the maximum inhibition zone diameter of 15.48 ± 0.07 mm. Baker’s yeast S cerevisiae biomass and A. niger from moldy onions has not been previously explored as a source of extractible fungal chitosan. This study gives insight that S. cerevisiae and A. niger from agricultural or industrial wastes could be a potential biomass source for production of the chitosan biopolymer. The S. cerevisiae chitosan displayed effective antimicrobial properties against S aureus, indicating the viablitiy of S cerevisae as a resource for extraction of high-quality chitosan.

尽管广泛使用，但酿酒酵母尚未被广泛探索用于直接提取壳聚糖生物聚合物以用于抗菌应用。在我们的研究中，来自贝克酵母的酿酒酵母和来自发霉洋葱提取物的黑曲霉被研究作为壳聚糖的替代来源；和酿酒酵母壳聚糖测试了抗菌功效。将酿酒酵母壳聚糖的性质与发霉的洋葱壳聚糖和从虾壳中提取的虾壳聚糖进行了比较。测试从酿酒酵母中提取的壳聚糖对金黄色葡萄球菌的抗菌功效。使用含有乙酸钠的培养液，真菌壳聚糖的最大产量在第 4 天为 20.85 ± 0.35 mg/g 干酿酒酵母生物量，在第 12 天为 16.15 ± 0.95 mg/g 干黑曲霉生物量。使用傅里叶变换红外光谱法，发现从酿酒酵母和黑曲霉中提取的真菌壳聚糖样品的脱乙酰度 (DD%)分别为 63.4% 和 61.2%。在 2 g/L 的浓度下，S. cerevisiae壳聚糖的最大抑菌圈直径为 15.48 ± 0.07 mm。贝克酵母S cerevisiae生物质和A. niger从发霉的洋葱中提取的可提取真菌壳聚糖的来源以前没有被探索过。这项研究表明，来自农业或工业废物的酿酒酵母和黑曲霉可能是生产壳聚糖生物聚合物的潜在生物质来源。酿酒酵母壳聚糖对金黄色葡萄球菌表现出有效的抗菌特性，表明酿酒酵母作为提取优质壳聚糖的资源具有可行性。