Mixotrophy (MX, also called partial mycoheterotrophy) in plants is characterized by isotopic abundances that differ from those of autotrophs. Previous studies have evaluated mycoheterotrophy in MX plants associated with fungi of similar ecological characteristics, but little is known about the differences in the relative abundances of ¹³C and ¹⁵N in an orchid species that associates with several different mycobionts species. Since the chlorophyllous orchid Cremastra variabilis Nakai associates with various fungi with different ecologies, we hypothesized that it may change its relative abundances of ¹³C and ¹⁵N depending on the associated mycobionts. We investigated mycobiont diversity in the chlorophyllous orchid C. variabilis together with the relative abundance of ¹³C and ¹⁵N and morphological underground differentiation (presence or absence of a mycorhizome with fungal colonization). Rhizoctonias (Tulasnellaceae, Ceratobasidiaceae, Sebacinales) were detected as the main mycobionts. High differences in δ¹³C values (– 34.7 to – 27.4 ‰) among individuals were found, in which the individuals associated with specific Psathyrellaceae showed significantly high relative abundance of ¹³C. In addition, Psathyrellaceae fungi were always detected on individuals with mycorhizomes. In the present study, MX orchid association with non-rhizoctonia saprobic fungi was confirmed, and the influence of mycobionts on morphological development and on relative abundance of ¹³C and ¹⁵N was discovered. Cremastra variabilis may increase opportunities to gain nutrients from diverse partners, in a bet-hedging plasticity that allows colonization of various environmental conditions.

植物中的混合营养（MX，也称为部分真菌异养）的特征是同位素丰度与自养生物不同。先前的研究已经评估了与具有相似生态特征的真菌相关的 MX 植物中的真菌异养性，但对与几种不同真菌生物相关联的兰花物种中¹³ C 和¹⁵ N相对丰度的差异知之甚少。由于叶绿素兰花Cremastra variabilis Nakai 与各种不同生态的真菌相关联，我们假设它可能会改变其¹³ C 和^{15 的}相对丰度N 取决于相关的菌群。我们研究了叶绿素兰花C. variabilis中的真菌群落多样性以及¹³ C 和¹⁵ N的相对丰度以及地下形态分化（存在或不存在真菌定植的菌根）。丝核菌（Tulasnellaceae、Ceratobasidiaceae、Sebacinales）被检测为主要的真菌。发现个体间δ ¹³ C 值（– 34.7 至 – 27.4 ‰）存在很大差异，其中与特定 Psathyrellaceae 相关的个体显示出显着较高的¹³C. 此外，在有菌根的个体上总是能检测到 Psathyrellaceae 真菌。在本研究中，与非MX兰花协会-丝核菌真菌saprobic确认，和形态学发展和相对丰度mycobionts的影响¹³ C和¹⁵ n为发现的。Cremastra variabilis可能会增加从不同伙伴那里获得营养的机会，在赌注-对冲可塑性，允许在各种环境条件下定居。