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Characterization of multi-metal-resistant Serratia sp. GP01 for treatment of effluent from fertilizer industries
Archives of Microbiology ( IF 2.3 ) Pub Date : 2021-08-17 , DOI: 10.1007/s00203-021-02523-z
Hrudananda Sahoo 1 , Sushama Kumari 1 , Umesh Chandra Naik 1, 2
Affiliation  

The effluent generated from fertilizer plants in Paradeep in the coast of the Bay of Bengal is the major pollutant causing health hazard in the vicinity of the area with respect to plants, animals and microbes. Samples of effluent were found to contain heavy metals (mg L−1): Cr (100), Ni (36.975), Mn (68.673), Pb (20.133), Cu (74.44), Zn (176.716), Hg (5.358) and As (24.287) as analyzed by XRF. Indigenous bacterial strains were screened for chromate and multi-metal resistance to remediate the toxic pollutants. The isolated strain G1 was identified as Serratia sp. through 16S-rDNA sequence homology. The potent strain Serratia sp. GP01 treated with 100 mg L−1 of K2Cr2O7 has shown the efficacy of reducing 69.05 mg L−1 of Cr over 48 h of incubation. Further, presence of chromate reductase gene (ChR) in Serratia sp. confirmed the enzymatic reduction of Cr(VI). SEM–EDX and SEM mapping analysis revealed substantial biosorption of Cr and other heavy metals present in effluent by Serratia sp. GP01. Antioxidant enzymes such as catalase (72.15 U mL−1), SOD (57.14 U mL−1) and peroxidase (62.49 U mL−1) were found to be higher as compared to the control condition. FTIR study also revealed the role of N–H, O–H, C = C, C–H, C–O, C–N, and C = O functional groups of the cell surface of Serratia sp. treated with K2Cr2O7 and effluent from the fertilizer industry. Isolated strain Serratia sp. could be used for the detoxification of Cr(VI) and other heavy metals in fertilizer plant effluent.



中文翻译:

多金属抗性沙雷氏菌的表征。GP01 用于处理化肥行业的废水

孟加拉湾沿岸 Paradeep 的化肥厂产生的废水是对该地区附近植物、动物和微生物造成健康危害的主要污染物。流出物样品被发现含有重金属 (mg L −1 ):Cr (100)、Ni (36.975)、Mn (68.673)、Pb (20.133)、Cu (74.44)、Zn (176.716)、Hg (5.358)和通过 XRF 分析的 As (24.287)。筛选本地细菌菌株的铬酸盐和多金属抗性以修复有毒污染物。分离菌株G1被鉴定为沙雷氏菌属。通过 16S-rDNA 序列同源性。强效菌株Serratia sp。GP01 用 100 mg L −1的 K 2 Cr 2 O 7处理已经显示出在 48 小时的孵化过程中减少 69.05 mg L -1 Cr 的功效。此外,沙雷氏菌中存在铬酸盐还原酶基因 ( ChR ) 。证实了 Cr(VI) 的酶促还原。SEM-EDX 和 SEM 绘图分析表明,沙雷氏菌对流出物中存在的 Cr 和其他重金属有大量的生物吸附作用。GP01。与对照条件相比,发现抗氧化酶如过氧化氢酶 (72.15 U mL -1 )、SOD (57.14 U mL -1 ) 和过氧化物酶 (62.49 U mL -1 ) 更高。FTIR 研究还揭示了沙雷氏菌细胞表面 N-H、O-H、C = C、C-H、C-O、C-N 和 C = O 官能团的作用sp。用 K 2 Cr 2 O 7和化肥工业的废水处理。分离菌株Serratia sp。可用于化肥厂废水中 Cr(VI) 等重金属的解毒。

更新日期:2021-10-09
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