Evodiamine, an alkaloid component in the fruit of Evodia, has been shown to have biological functions such as antioxidant and anti-inflammatory. But whether evodiamine plays an improvement role on mastitis has not been studied. To investigate the effect and mechanism of evodiamine on lipopolysaccharide (LPS)-induced mastitis was the purpose of this study. In animal experiments, the mouse mastitis model was established by injecting LPS into the canals of the mammary gland. The results showed that evodiamine could significantly relieve the pathological injury of breast tissue and the production of pro-inflammatory cytokines and inhibit the activation of inflammation-related pathways such as AKT, NF-κB p65, ERK1/2, p38, and JNK. In cell experiments, the mouse mammary epithelial cells (mMECs) were incubated with evodiamine for 1 h and then stimulated with LPS. Next, pro-inflammatory mediators and inflammation-related signal pathways were detected. As expected, our results showed that evodiamine notably ameliorated the inflammatory reaction and inhibit the activation of related signaling pathways of mMECs. All the results suggested that evodiamine inhibited inflammation by inhibiting the phosphorylation of AKT, NF-κBp65, ERK1/2, p38, and JNK thus the LPS-induced mastitis was ameliorated. These findings suggest that evodiamine maybe a potential drug for mastitis because of its anti-inflammatory effects.

吴茱萸碱吴茱萸果实中的一种生物碱成分，已被证明具有抗氧化和抗炎等生物学功能。但吴茱萸碱是否对乳腺炎有改善作用尚未研究。本研究旨在探讨吴茱萸碱对脂多糖（LPS）诱导的乳腺炎的作用及其机制。在动物实验中，通过将LPS注入乳腺管建立小鼠乳腺炎模型。结果表明吴茱萸碱可显着减轻乳腺组织的病理损伤和促炎细胞因子的产生，抑制炎症相关通路如AKT、NF-κB p65、ERK1/2、p38和JNK的激活。在细胞实验中，小鼠乳腺上皮细胞 (mMEC) 与吴茱萸碱一起孵育 1 小时，然后用 LPS 刺激。接下来，检测促炎介质和炎症相关信号通路。正如预期的那样，我们的结果表明吴茱萸碱显着改善了炎症反应并抑制了 mMECs 相关信号通路的激活。所有结果表明吴茱萸碱通过抑制 AKT、NF-κBp65、ERK1/2、p38 和 JNK 的磷酸化来抑制炎症，从而改善 LPS 诱导的乳腺炎。这些发现表明吴茱萸碱可能是一种潜在的乳腺炎药物，因为它具有抗炎作用。所有结果表明吴茱萸碱通过抑制 AKT、NF-κBp65、ERK1/2、p38 和 JNK 的磷酸化来抑制炎症，从而改善 LPS 诱导的乳腺炎。这些发现表明吴茱萸碱可能是一种潜在的乳腺炎药物，因为它具有抗炎作用。所有结果表明吴茱萸碱通过抑制 AKT、NF-κBp65、ERK1/2、p38 和 JNK 的磷酸化来抑制炎症，从而改善 LPS 诱导的乳腺炎。这些发现表明吴茱萸碱可能是一种潜在的乳腺炎药物，因为它具有抗炎作用。