An obligate anaerobic bacterial BAD-10 ^T was isolated from anaerobic acetochlor-degrading sludge. The strain was Gram-stain negative, curved rod-shaped, non-motile and non-spore-forming. Growth was observed in PYT medium at pH 6.0–9.0 (optimum, pH 7.5), at 25–47 °C (37 °C) and with 0–1.0% NaCl (w/v, 0%). Strain BAD-10 ^T could degrade acetochlor. The major fermentation products from peptone-yeast (PY) medium were acetate and butyrate. The predominant cellular fatty acids were iso-C_15:0 FAME, anteiso-C_15:0 FAME and C_16:0 FAME. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain BAD-10 ^T showed closest affiliation to Proteiniclasticum ruminis D3RC-2 ^T, with a sequence similarity of 97.6%. Genome sequencing revealed a genome size of 2,983,986 bp, a G + C content of 51.4 mol% and protein-coding genes of 3,102. The average nucleotide identity and in silico DNA–DNA hybridization values between strain BAD-10 ^T and Proteiniclasticum ruminis D3RC-2 ^T were 71.0% and 20.4%, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, physiological and phylogenetic evidence, strain BAD-10 ^T represents a novel species in the genus Proteiniclasticum, for which the name Proteiniclasticum sediminis sp. nov. is proposed. Strain BAD-10 ^T (= CCTCC AB 2021091 ^T = KCTC 25288 ^T) is the type strain of the proposed novel species.

从厌氧乙草胺降解污泥中分离出专性厌氧细菌 BAD-10  ^T。该菌株为革兰氏染色阴性、弯曲的杆状、非运动且不形成孢子。在 pH 6.0–9.0（最佳，pH 7.5）、25–47 °C (37 °C) 和 0–1.0% NaCl（w/v，0%）的 PYT 培养基中观察到生长。菌株 BAD-10  ^T可降解乙草胺。蛋白胨-酵母（PY）培养基的主要发酵产物是乙酸盐和丁酸盐。主要的细胞脂肪酸是异 C _15:0 FAME、反异 C _15:0 FAME 和 C _16:0 FAME。基于 16S rRNA 基因序列的系统发育分析表明，BAD-10  ^T菌株与Proteiniclasticum ruminis 的亲缘关系最近D3RC-2  ^T，序列相似度为 97.6%。基因组测序显示基因组大小为 2,983,986 bp，G + C 含量为 51.4 mol%，蛋白质编码基因为 3,102。菌株BAD-10  ^T和Proteiniclastum ruminis D3RC-2  ^T之间的平均核苷酸同一性和计算机DNA-DNA 杂交值分别为71.0% 和20.4%，低于物种分化的标准阈值。根据表型、生理学和系统发育证据，菌株 BAD-10  ^T代表Proteiniclasticum属中的一个新物种，其名称为Proteiniclasticum sediminis sp。十一月 被提议。应变 BAD-10  ^T(= CCTCC AB 2021091  ^T  = KCTC 25288  ^T ) 是提议的新物种的类型菌株。