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Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge

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Abstract

An obligate anaerobic bacterial BAD-10 T was isolated from anaerobic acetochlor-degrading sludge. The strain was Gram-stain negative, curved rod-shaped, non-motile and non-spore-forming. Growth was observed in PYT medium at pH 6.0–9.0 (optimum, pH 7.5), at 25–47 °C (37 °C) and with 0–1.0% NaCl (w/v, 0%). Strain BAD-10 T could degrade acetochlor. The major fermentation products from peptone-yeast (PY) medium were acetate and butyrate. The predominant cellular fatty acids were iso-C15:0 FAME, anteiso-C15:0 FAME and C16:0 FAME. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain BAD-10 T showed closest affiliation to Proteiniclasticum ruminis D3RC-2 T, with a sequence similarity of 97.6%. Genome sequencing revealed a genome size of 2,983,986 bp, a G + C content of 51.4 mol% and protein-coding genes of 3,102. The average nucleotide identity and in silico DNA–DNA hybridization values between strain BAD-10 T and Proteiniclasticum ruminis D3RC-2 T were 71.0% and 20.4%, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, physiological and phylogenetic evidence, strain BAD-10 T represents a novel species in the genus Proteiniclasticum, for which the name Proteiniclasticum sediminis sp. nov. is proposed. Strain BAD-10 T (= CCTCC AB 2021091 T = KCTC 25288 T) is the type strain of the proposed novel species.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strain BAD-10 T are MW491274 and JAGSCS000000000, respectively.

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Funding

This work was supported by the Key research and development project of science and technology development plan of Jilin Province (20180201062NY).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People’s Republic of China

    Junwei Liu, Yixuan Bao, Xuan Zhang, Kaiyun Zhang & Jian He

  2. Institute of Agricultural Environment and Resources Research, Jilin Academy of Agricultural Sciences, Changchun, Jilin, 130033, People’s Republic of China

    Shuaimin Chen & Haiyan Wu

Authors
  1. Junwei Liu
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  2. Yixuan Bao
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  3. Xuan Zhang
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  4. Kaiyun Zhang
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  5. Shuaimin Chen
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  6. Haiyan Wu
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  7. Jian He
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Contributions

Conceptualization, funding acquisition, and supervision: HW and JH; Laboratory work, data analysis and writing-original draft: JL; Writing-review and editing: YB, XZ, KZ and SC. All authors read and approved the final manuscript.

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Correspondence to Haiyan Wu or Jian He.

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Liu, J., Bao, Y., Zhang, X. et al. Proteiniclasticum sediminis sp. nov., an obligate anaerobic bacterium isolated from anaerobic sludge. Antonie van Leeuwenhoek 114, 1541–1549 (2021). https://doi.org/10.1007/s10482-021-01620-9

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  • DOI: https://doi.org/10.1007/s10482-021-01620-9

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