A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4,118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The first ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (P40, nt: 20-1,096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2,683, P100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic fleck-associated tombusvirus (GCFaV-1, Accession No. QKE30557) had the highest sequence identity (55.5%) with ParV2. GCFaV-1 also shares 59.2% RdRP and 34.9% CP amino acid sequence identities with ParV2. Sequence comparisons and phylogenetic analysis of RdRP suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.

中文翻译：

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从Paris polyphylla var. 中分离出的一种新型Tombusviridae 物种的表征。云南.

从患病的巴黎多叶变种中分离出一种新型病毒，巴黎病毒 2 (ParV2)。yunnanensis，并对其完整的基因组序列进行了测定和分析。ParV2 是一种正链单链 RNA (+ssRNA) 病毒，基因组大小为 4,118 个核苷酸。ParV2 基因组包含六个假定的开放阅读框 (ORF)，可编码预测分子量为 40.14、100.26、7.31、7.85、26.09 和 8.77 kDa 的蛋白质。ParV2 的第一个 ORF (ORF1) 编码 40.14 kDa (P40, nt: 20-1,096) 的推定蛋白质，而包含 GDD 基序的第二个 ORF (ORF2, 888 aa) 编码高度保守的依赖于 RNA 的 RNA 聚合酶蛋白。 RdRP, nt:20-2,683, P100, 100.26 kDa) 的蘑菇病毒科病毒。多序列比对分析表明ParV2的完整基因组序列共享31.7-55。与 Tombusviridae 家族中的病毒具有 5% 的核苷酸序列同一性。生姜褪绿斑点相关墓穴病毒（GCFaV-1，登录号 QKE30557）与 ParV2 具有最高的序列同一性（55.5%）。GCFaV-1 还与 ParV2 共享 59.2% RdRP 和 34.9% CP 氨基酸序列同一性。RdRP 的序列比较和系统发育分析表明 ParV2 是 Tombusviridae 家族的一个新成员，其已知的最近亲属是 GCFaV-1。