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Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis

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Abstract

A novel virus, Paris virus 2 (ParV2), was isolated from diseased Paris polyphylla var. yunnanensis, and its complete genome sequence was determined and analyzed. ParV2 is a positive-sense single-stranded RNA (+ssRNA) virus with a genome size of 4,118 nucleotides. The ParV2 genome contains six putative open reading frames (ORFs) that encode proteins with predicted molecular weights of 40.14, 100.26, 7.31, 7.85, 26.09, and 8.77 kDa. The first ORF (ORF1) of ParV2 encodes a putative protein of 40.14 kDa (P40, nt: 20-1,096), whiles the second ORF (ORF2, 888 aa) containing the GDD motif encodes the highly conserved RNA-dependent RNA polymerase protein (RdRP, nt:20-2,683, P100, 100.26 kDa) of viruses in the family Tombusviridae. Multiple sequence alignments analysis showed that the complete genome sequence of ParV2 shares 31.7-55.5% nucleotide sequence identities with viruses in the family Tombusviridae. Ginger chlorotic fleck-associated tombusvirus (GCFaV-1, Accession No. QKE30557) had the highest sequence identity (55.5%) with ParV2. GCFaV-1 also shares 59.2% RdRP and 34.9% CP amino acid sequence identities with ParV2. Sequence comparisons and phylogenetic analysis of RdRP suggested that ParV2 is a novel member of the family Tombusviridae, and its closest known relative is GCFaV-1.

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Acknowledgements

This work was funded by the National Natural Science Foundation (Grant No. 81860774).

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Author notes

  1. Lu Chen and Rex Frimpong Anane equally contributed to this study.

Authors and Affiliations

  1. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Lu Chen, Zhe Wang, Like Gao & Mingfu Zhao

  2. Research and Development Center for Health Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 495 Jinhei Road, Kunming, 650201, Yunnan, China

    Lu Chen, Zhe Wang, Zeli Chen, Like Gao, Guosong Wen & Mingfu Zhao

  3. Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  4. State Key Laboratory for Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  5. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen & Guosong Wen

Authors
  1. Lu Chen
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  2. Rex Frimpong Anane
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Corresponding authors

Correspondence to Guosong Wen or Mingfu Zhao.

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Chen, L., Anane, R.F., Wang, Z. et al. Characterization of a novel Tombusviridae species isolated from Paris polyphylla var. yunnanensis. Arch Virol 166, 3199–3205 (2021). https://doi.org/10.1007/s00705-021-05191-y

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  • DOI: https://doi.org/10.1007/s00705-021-05191-y

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