Seeking simple, green, and inexpensive preparation methods and materials of enzyme mimics has always been highly desirable. In this paper, a nanocomposite with laccase activity was successfully fabricated by using a simple self-association method with copper and tannic acid. Characterizations through SEM, XRD, FTIR, and XPS showed that the Cu-TA hybrid composites had good spherical in shape with a diameter of about 20 nm and three different valence Cu ions (Cu²⁺, Cu⁺, and Cu⁰). The catalytic activity was displayed against typical oxidation substrate of laccases, 2,4-DP. Compared to natural laccases, Cu-TA composites were able to better tolerate changes of pH, temperature, ionic strength, and storage conditions. The Cu-TA composites could remove malachite green (MG) from aqueous solutions effectively. The removal efficiency of MG was optimized using a series of batch tests with single-factor experiment design. Under the optimum conditions, the removal efficiency of MG still retained about 90% after 3 times cycles. Therefore, the Cu-TA composites, as a laccases-like nanozyme, have excellent developing potential in wastewater treatment due to the low-cost of TA and simplicity of preparation.

寻求简单、绿色、廉价的酶模拟物制备方法和材料一直是人们非常渴望的。在本文中，通过使用简单的自缔合方法与铜和单宁酸成功制备了具有漆酶活性的纳米复合材料。通过 SEM、XRD、FTIR 和 XPS 表征表明，Cu-TA 杂化复合材料具有良好的球形，直径约为 20 nm，具有三种不同价态的 Cu 离子（Cu ²⁺、Cu ⁺和 Cu ⁰）。对漆酶的典型氧化底物 2,4-DP 显示出催化活性。与天然漆酶相比，Cu-TA 复合材料能够更好地耐受 pH、温度、离子强度和储存条件的变化。Cu-TA复合材料可以有效去除水溶液中的孔雀石绿（MG）。MG 的去除效率通过一系列单因素实验设计的批量测试进行了优化。在最佳条件下，3次循环后MG的去除效率仍保持在90%左右。因此，Cu-TA复合材料作为一种类漆酶纳米酶，由于TA的成本低和制备简单，在废水处理中具有良好的发展潜力。