In this study, the combined techniques of macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for the separation of steroidal saponins from the stems and leaves of Paris polyphylla. Eight types of macroporous resins were examined using adsorption and desorption tests. Resin HPD-600 showed the maximum effectiveness and thus was selected for the first cleaning-up, in which 30% ethanol was used to remove the undesired constituents and 90% ethanol was used to elute the targets. Paris saponin II (36.2 mg) and dioscin (14.0 mg) were obtained from 500.5 mg of the extracts after purification by preparative high-performance liquid chromatography, with purities above 95% and yields of 81.87 and 72.89%, respectively. These results suggest that combined strategy is a useful and economic method to enrich and purify steroidal saponins from the stems and leaves of P. polyphylla.

本研究采用大孔树脂柱色谱法和制备型高效液相色谱法相结合的技术，从七叶树茎叶中分离甾体皂苷。. 使用吸附和解吸试验检测了八种类型的大孔树脂。树脂 HPD-600 显示出最大的有效性，因此被选为第一次净化，其中使用 30% 的乙醇去除不需要的成分，使用 90% 的乙醇洗脱目标。提取物500.5mg经制备型高效液相色谱纯化后得到巴黎皂苷II(36.2mg)和薯蓣皂苷(14.0mg)，纯度分别为95%以上，收率分别为81.87%和72.89%。这些结果表明，联合策略是一种从多叶植物茎叶中富集和纯化甾体皂苷的有效且经济的方法。