Skip to main content
SpringerLink
Log in

Combined Application of Macroporous Resins and Preparative High-performance Liquid Chromatography for the Separation of Steroidal Saponins from Stems and Leaves of Paris polyphylla

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

In this study, the combined techniques of macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for the separation of steroidal saponins from the stems and leaves of Paris polyphylla. Eight types of macroporous resins were examined using adsorption and desorption tests. Resin HPD-600 showed the maximum effectiveness and thus was selected for the first cleaning-up, in which 30% ethanol was used to remove the undesired constituents and 90% ethanol was used to elute the targets. Paris saponin II (36.2 mg) and dioscin (14.0 mg) were obtained from 500.5 mg of the extracts after purification by preparative high-performance liquid chromatography, with purities above 95% and yields of 81.87 and 72.89%, respectively. These results suggest that combined strategy is a useful and economic method to enrich and purify steroidal saponins from the stems and leaves of P. polyphylla.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig.4
Fig.5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Chinese Pharmacopoeia Commission (2015) Chinese pharmacopoeia. Part 1. China Medical Science Press, Beijing, p 260

    Google Scholar 

  2. Wang FF, Ma X, Li ZB, Yuan YL, Qu LP (2017) Pharm J Chin PLA 33:315–318

    Google Scholar 

  3. Jing SS, Wang Y, Li X, Man SL, Gao WY (2017) Nat Prod Res 31:660–666

    Article  CAS  Google Scholar 

  4. Zhang C, Li CY, Jia XJ, Wang K, Tu YB, Wang RC, Liu KC, Lu T, He CW (2019) Molecules 24:1–17

    Google Scholar 

  5. Xie ZZ, Li MM, Deng PF, Wang S, Wang L, Lu XP, Hu LB, Chen Z, Jie HY, Wang YF, Liu XX, Liu Z (2017) Chem Biol Interact 25:1–9

    Article  Google Scholar 

  6. Qin XJ, Ni W, Chen CX, Liu HY (2018) J Ethnopharmacol 224:134–139

    Article  CAS  Google Scholar 

  7. Chen MH, Ye K, Zhang BY, Xin Q, Li P, Kong AN, Wen XD, Yang J (2019) Pharmacol Res 139:273–285

    Article  CAS  Google Scholar 

  8. Zhang LL, Man SL, Wang YS, Liu J, Liu Z, Yu P, Gao WY (2016) Chem Biol Interact 253:125–133

    Article  CAS  Google Scholar 

  9. Yang M, Zou J, Zhu HM, Liu SL, Wang H, Bai P, Xiao X (2015) Oncol Rep 33:2190–2198

    Article  CAS  Google Scholar 

  10. Xiao X, Zou J, Bui-Nguyen TM, Bai P, Gao LB, Liu JS, Liu SL, Xiao JG, Chen XL, Zhang XM, Wang H (2012) BioSci Trends 6:201–211

    Article  CAS  Google Scholar 

  11. Tao XF, Yin LH, Xu LN, Peng JY (2018) Pharmacol Res 137:259–269

    Article  CAS  Google Scholar 

  12. Zhao XR, Yin LH, Fang LL, Xu LN, Sun PY, Xu M, Liu KX, Peng JY (2018) Int Immunopharmacol 65:458–469

    Article  CAS  Google Scholar 

  13. Qin XJ, Yu MY, Ni W, Yan H, Chen CX, Cheng YC, He L, Liu HY (2016) Phytochemistry 121:20–29

    Article  CAS  Google Scholar 

  14. Yin W, Song ZR, Liu JQ, Zhang GS (2015) J Chin Med Mater 38:1875–1878

    CAS  Google Scholar 

  15. Zhong JL, Muhammad N, Gu YC, Yan WD (2019) J Food Eng 243:82–88

    Article  CAS  Google Scholar 

  16. Liu Z, Wang JY, Gao WY, Man SL, Wang Y, Liu CX (2013) Pharm biol 51:899–905

    Article  CAS  Google Scholar 

  17. Chen HG, Liang Q, Zhou X, Wang XY (2019) J Sep Sci 42:650–661

    Article  CAS  Google Scholar 

  18. Mota MI, Barbosa FS, Pinto PR, Ribeiro AM, Ferreira AF, Loureiro JM, Rodrigues AE (2019) Sep Purif Technol 217:108–117

    Article  CAS  Google Scholar 

  19. Wu YF, Zhang L, Mao JW, Liu SW, Huang J, You YR, Mei LH (2016) J Chromatogr A 1028:231–236

    Google Scholar 

  20. Li HJ, Hou GG, Li YY, Zhao F, Cong W, Wang CH (2018) J Sep Sci 41:3918–3924

    Article  CAS  Google Scholar 

  21. Li F, Sun XY, Li XW, Yang T, Qi LW (2017) J Chromatogr B 1040:186–191

    Article  CAS  Google Scholar 

  22. Qu LP, Xin HL, Su YH, Zheng GY, Ling CQ (2012) J Sep Sci 35:883–892

    Article  CAS  Google Scholar 

  23. Yang DJ, Lu TJ, Hwang LS (2003) J Agric Food Chem 51:6438–6444

    Article  CAS  Google Scholar 

  24. Liang YN, Fan RT, Wang Z, Liu SJ, Ge QP, Sang YY, Tang ZS (2017) China Pharm 28:4868–4871

    Google Scholar 

  25. Nie J, Li ZS, Tong JM, Liu CN, Liu KM, Liu YP (2018) China Pharm 29:322–326

    Google Scholar 

  26. Zou R, Yan XM (2020) J Cap Nor Uni (Nat. Sci. Edi.) 41:69–75

    Google Scholar 

  27. Vienna S, Ketut AI, Sri P, Tjandrawati M, Muhammad I, Neng FK, Heni R (2017) J Food Sci Technol 54:3349–3357

    Article  Google Scholar 

Download references

Acknowledgements

This study was financially supported by Key Research and Development Plan of Yunnan Province–Science and Technology Project (2017IB007).

Author information

Authors and Affiliations

  1. Characteristic Plants Research and Development Center, Botanee Research Institute, Shanghai Jiyan Bio-Pharmaceutical Development Co., Ltd., 10/F, Unit B, North Zone, e-Link World, No.999 Huaxu Road, Qingpu District, Shanghai, 201702, China

    Feifei Wang, Xiao Ma & Liping Qu

  2. Yunnan Botanee Bio-Technology Group Co., Ltd, Yunnan, 650106, China

    Feifei Wang, Xiao Ma & Liping Qu

Authors
  1. Feifei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liping Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liping Qu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, F., Ma, X. & Qu, L. Combined Application of Macroporous Resins and Preparative High-performance Liquid Chromatography for the Separation of Steroidal Saponins from Stems and Leaves of Paris polyphylla. Chromatographia 84, 917–925 (2021). https://doi.org/10.1007/s10337-021-04073-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10337-021-04073-4

Keywords

Search

Navigation