The hard-end of pear fruit might be a disorder of the environmental abiotic stress, which is mainly caused by the reduced water intake of the fruit resulting from climate drought. To explore the factors that cause fruit hard-end, two rootstocks of Pyrus betulaefolia and P. bretschneideri with different root absorptive capacity of water and nutrients were used as base stock of ‘Whangkeumbae’ (P. pyrifolia) pear fruit. The results showed that the incidence of hard-end fruit in ‘Whangkeumbae’ pear trees using rootstock with lower rate of water absorptive increased, indicating that this physiological disorder was related with water deficiency. PpNAC187 was selected from RNA-Seq carried out on the calyx end of with and without the hard-end symptom of different rootstocks. The transgenic tobacco overexpressing PpNAC187 showed higher tolerance with drought and salt stress, which might suggest that PpNAC187 acts an important role during the hard-end occurring caused by drought or salt stress.

梨果实硬端可能是环境非生物胁迫失调，主要是气候干旱导致果实吸水量减少所致。为探究导致果实硬端的因素，以根部对水分和养分吸收能力不同的两种砧木Pyrus betulaefolia和P. bretschneideri作为'Whangkeumbae'（P. pyrifolia）梨果实的基础原料。结果表明，使用吸水率较低的砧木'Whangkeumbae'梨树的硬端果实发生率增加，表明这种生理障碍与缺水有关。PpNAC187是从有和没有不同砧木硬端症状的花萼末端进行的RNA-Seq中选择的。过表达PpNAC187的转基因烟草对干旱和盐胁迫表现出更高的耐受性，这可能表明PpNAC187在干旱或盐胁迫引起的硬端发生过程中发挥了重要作用。