Abstract
The hard-end of pear fruit might be a disorder of the environmental abiotic stress, which is mainly caused by the reduced water intake of the fruit resulting from climate drought. To explore the factors that cause fruit hard-end, two rootstocks of Pyrus betulaefolia and P. bretschneideri with different root absorptive capacity of water and nutrients were used as base stock of ‘Whangkeumbae’ (P. pyrifolia) pear fruit. The results showed that the incidence of hard-end fruit in ‘Whangkeumbae’ pear trees using rootstock with lower rate of water absorptive increased, indicating that this physiological disorder was related with water deficiency. PpNAC187 was selected from RNA-Seq carried out on the calyx end of with and without the hard-end symptom of different rootstocks. The transgenic tobacco overexpressing PpNAC187 showed higher tolerance with drought and salt stress, which might suggest that PpNAC187 acts an important role during the hard-end occurring caused by drought or salt stress.
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References
Bach-Pages M, Preston GM (2018) Methods to quantify biotic-induced stress in plants. Methods Mol Biol 1734:241–255
Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran LS, Yamaguchi-Shinozaki K, Shinozaki K (2004) A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J 39:863–876
Guan LM, Scandalios JG (2000) Hydrogen-peroxide-mediated catalase gene expression in response to wounding. Free Radic Biol Med 28:1182–1190
Kenneth JL, Thomas DS (2002) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25:402–408
Li Z, Wang R (2011) Effects of leaf and fruit water potential and photosynthetic traits on huangding disease of Whangkeumbae fruit. North Hortic 4:29–32
Li M, Cheng C, Zhang X, Zhou S, Li L, Yang S (2019a) Overexpression of pear (Pyrus pyrifolia) CAD2 in tomato affects lignin content. Molecules 24:2595
Li M, Cheng C, Zhang X, Zhou S, Wang C, Ma C, Yang S (2019b) PpNAC187 enhances lignin synthesis in ‘Whangkeumbae’ pear (Pyrus pyrifolia) ‘hard-end’fruit. Molecules 24:4338
Lu G, Li Z, Zhang X, Wang R, Yang S (2015) Expression analysis of lignin associated genes in hard end pear (Pyrus pyrifolia Whangkeumbae) and its response to cal-cium chloride treatment conditions. J Plant Growth Regul 34:251–262
Ma C, Li D, Wang R (2014) Microscopic observation of the ‘hard-end disorder’ on the pulp tissue of ‘Hwangkumbae’ pear. North Hortic 13:109–113
Ohlendorf BL (1999) Integrated pest management for apples & pears. University of California, Berkeley
Raese JT (1994) Fruit disorders, mineral composition and tree performance influenced by rootstocks of ‘Anjou’ pears. Acta Hortic. https://doi.org/10.17660/ActaHortic.1994.367.56
Raese JT, Drake SR (1993) Effects of preharvest calcium sprays on apple and pear quality. J Plant Nutr 16:1807–1819
Raese JT, Drake SR (1996) Yield increased and fruit disorders decreased with repeated annual calcium sprays on ‘Anjou’ pears. J Tree Fruit Prod 1:51–59
Raese JT, Drake SR, Staiff DC (1995) Influence of different calcium materials and spray timing on mineral composition, yield, fruit quality, and control of fruit disorders of ‘anjou’ pears. J Plant Nutr 18:823–838
Wang Y, Zhang X, Wang Y, Yang S, Qu H (2018) The changes of intracellular calcium concentration and distribution in the hard end pear ( Pyrus pyrifolia cv. ‘Whangkeumbae’) fruit. Cell Calcium 71:15–23
Welsh M (1979) Pear fruit blemishes and defects. British Columbia Grower Magazine 1:10–12
Yamamoto T, Watanabe S (1982) Water potentials and water fluxes into fruit at the initial time of development of hard end disorder in “Bartlett” pear. J Jpn Soc Hortic Sci 51:395–404
Yan Y, Gong W, Yang W, Wan Y, Chen X, Chen Z, Wang L (2010) Seed treatment with uniconazole powder improves soybean seedling growth under shading by corn in relay strip in-tercropping system. Plant Prod Sci 72:367–374
Yu X, Liu Y, Wang S, Tao Y, Wang Z, Shu Y, Peng H, Mijiti A, Wang Z, Zhang H, Ma H (2016) CarNAC4, a NAC-type chickpea transcription factor conferring enhanced drought and salt stress tolerances in Arabidopsis. Plant Cell Rep 35:613–627
Zhang G, Huang S, Zhang C, Li D, Qi J (2020) Overexpression of CcNAC1 gene promotes early flowering and enhances drought tolerance of jute (Corchorus capsularis L.). Protoplasma 258:337–345
Zheng X, Chen B, Lu G, Han B (2009) Overexpression of a NAC transcription factor enhances rice drought and salt tolerance. Biochem Biophys Res Commun 379:985–989
Acknowledgements
The authors would like to thank the technical support from central laboratory in Qingdao agricultural university.
Funding
This work was funded by the Project for Improving Agriculture Varieties of Shandong Province in China (2019LZGC008), Agricultural science and technology fund of Shandong Province (2019YQ015), the Project of Shandong Natural Science Foundation (ZR2017MC006), the Breeding Plan of Shandong Provincial Qingchuang Research Team (2019), and Scientific Research Funds for High-level Personnel of Qingdao Agricultural University (1119046).
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Conceptualization, CM, and CC; data curation, ML, SY, XZ, YZ, QQ, and YZ; formal analysis, ML, SY, XZ, YZ, QQ, YZ, and CC; writing—original draft, CM, YZ, and CC; writing—review & editing, YZ and CC. All authors have read and agreed to the published version of the manuscript.
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Ma, C., Li, M., Yang, S. et al. The Role of PpNAC187 in Pear Drought and Salt Stress. J Plant Growth Regul 41, 2570–2578 (2022). https://doi.org/10.1007/s00344-021-10463-7
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DOI: https://doi.org/10.1007/s00344-021-10463-7