This article aims to present a simple and sensitive, HPLC–UV method, which was developed to determine carnosol in human plasma samples. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25 °C with gradient elution of the mobile phase consisting of methanol–water (2% o-phosphoric acid) at flow rate 1.2 mL/min. The analyte was detected at 230 nm by UV detector. The retention time of carnosol is 3.40 ± 0.01 min. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 1–20 ng/mL with the correlation coefficient of 0.9942. The proposed method was applied successfully to the analysis of carnosol in spiked human plasma with good recovery as 96.4% and the precision of the method was determined by intra day and interday assays with the highest RSD % values 5.71. The method successfully applied to a pharmacokinetic study with determination of C_max, t_max, t_1/2 and AUC, by administration of carnosol to a healthy volunteer.

本文旨在介绍一种简单而灵敏的 HPLC-UV 方法，该方法用于测定人血浆样品中的鼠尾草酚。色谱分离是使用 C18 柱（150 mm × 4.6 mm × 5 μm）在 25 °C 下用甲醇-水（2% o-磷酸），流速为 1.2 mL/min。通过紫外检测器在 230 nm 处检测分析物。鼠尾草酚的保留时间为 3.40 ± 0.01 分钟。这种目前开发的方法根据 ICH 标准通过评估特异性、线性、精密度、准确度和稳健性进行了验证。确定该方法在 1–20 ng/mL 的浓度范围内呈线性，相关系数为 0.9942。所提出的方法成功应用于加标人血浆中鼠尾草酚的分析，回收率高达 96.4%，该方法的精密度由日内和日间测定确定，最高 RSD % 值为 5.71。该方法成功应用于药代动力学研究，测定C _max , t _max , t_1/2和 AUC，通过向健康志愿者施用鼠尾草醇。