Abstract
This article aims to present a simple and sensitive, HPLC–UV method, which was developed to determine carnosol in human plasma samples. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25 °C with gradient elution of the mobile phase consisting of methanol–water (2% o-phosphoric acid) at flow rate 1.2 mL/min. The analyte was detected at 230 nm by UV detector. The retention time of carnosol is 3.40 ± 0.01 min. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 1–20 ng/mL with the correlation coefficient of 0.9942. The proposed method was applied successfully to the analysis of carnosol in spiked human plasma with good recovery as 96.4% and the precision of the method was determined by intra day and interday assays with the highest RSD % values 5.71. The method successfully applied to a pharmacokinetic study with determination of Cmax, tmax, t1/2 and AUC, by administration of carnosol to a healthy volunteer.
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This study is financially supported by the Scientific Research Projects Units of Bezmialem Vakıf University (Project No: 20200203).
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Ceylan, B., Tırıs, G. & Tekkeli, S.E.K. A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study. Chromatographia 84, 855–860 (2021). https://doi.org/10.1007/s10337-021-04069-0
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DOI: https://doi.org/10.1007/s10337-021-04069-0