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A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study

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Abstract

This article aims to present a simple and sensitive, HPLC–UV method, which was developed to determine carnosol in human plasma samples. Chromatographic separation was achieved with C18 column (150 mm × 4.6 mm × 5 μm), at 25 °C with gradient elution of the mobile phase consisting of methanol–water (2% o-phosphoric acid) at flow rate 1.2 mL/min. The analyte was detected at 230 nm by UV detector. The retention time of carnosol is 3.40 ± 0.01 min. This currently developed method was validated according to ICH criteria by evaluating the specificity, linearity, precision, accuracy and robustness. The method was determined to be linear in a concentration range of 1–20 ng/mL with the correlation coefficient of 0.9942. The proposed method was applied successfully to the analysis of carnosol in spiked human plasma with good recovery as 96.4% and the precision of the method was determined by intra day and interday assays with the highest RSD % values 5.71. The method successfully applied to a pharmacokinetic study with determination of Cmax, tmax, t1/2 and AUC, by administration of carnosol to a healthy volunteer.

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Acknowledgements

This study is financially supported by the Scientific Research Projects Units of Bezmialem Vakıf University (Project No: 20200203).

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Authors and Affiliations

  1. Department of Pharmacognosy and Natural Products Chemistry, Institute of Health Sciences, Bezmialem Vakif University, Fatih, Istanbul, Turkey

    Burhan Ceylan

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Bezmialem Vakif University, Fatih, 34093, Istanbul, Turkey

    Gizem Tırıs & S. Evrim Kepekci Tekkeli

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  1. Burhan Ceylan
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  2. Gizem Tırıs
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  3. S. Evrim Kepekci Tekkeli
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Correspondence to S. Evrim Kepekci Tekkeli.

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All procedure performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Bezmialem Vakıf University approved by the Clinical Trials Ethic Commitee (No: 24/23).

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Ceylan, B., Tırıs, G. & Tekkeli, S.E.K. A New HPLC Method with UV Detection for the Determination of Carnosol in Human Plasma and Application to a Pharmacokinetic Study. Chromatographia 84, 855–860 (2021). https://doi.org/10.1007/s10337-021-04069-0

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