Monatshefte für Chemie - Chemical Monthly ( IF 1.7 ) Pub Date : 2021-08-06 , DOI: 10.1007/s00706-021-02812-9 Flaudiner Gomes de Moura Junior 1 , William Barros Veloso 1 , José Antonio de Oliveira Junior 1 , Iranaldo Santos da Silva 1 , Luiza Maria Ferreira Dantas 1 , Heinz-Bernhard Kraatz 2
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In this paper, a composite based on chitosan and cobalt phthalocyanine immobilized onto a glassy carbon electrode is presented for the sensitive determination of sulfanilamide in pharmaceutical and urine samples. The modification performed on the electrode surface, by the deposition of this composite, exhibits an irreversible anodic peak at 0.97 V vs. Ag/AgCl, KClsat in phosphate buffer at pH 7.00 for sulfanilamide. Results show that peak potential is influenced linearly by the pH of the supporting electrolyte, exhibiting a slope of 0.054 V/pH. Using differential pulse voltammetry, detection of sulfanilamide was possible in a linear range from 1.00 to 53.00 μmol dm−3 with a detection limit and quantification limit of 0.27 and 0.91 μmol dm−3, respectively. Importantly, the sensor showed practical applications as an analytical tool for the quantification of sulfanilamide in pharmaceutical formulations and urine samples. The electrochemical approach detailed here has been validated to drug samples using a spectrophotometric method. The method proposed presents good linearity, low quantification and detection limits being of excellent applicability in pharmaceutical and urine samples.
Graphic abstract
中文翻译：
使用钴酞菁壳聚糖复合物伏安法测定磺胺
在本文中，提出了一种基于壳聚糖和钴酞菁的复合物固定在玻碳电极上，用于灵敏测定药物和尿液样品中的磺胺。由该复合物的沉积在电极表面上进行的，该变形例中，表现出0.97 V，相对于银/氯化银的不可逆阳极峰，氯化钾坐在在磷酸盐缓冲液为pH为磺胺7.00。结果表明，峰值电位受支持电解质的 pH 值线性影响，斜率为 0.054 V/pH。使用差分脉冲伏安法，可以在 1.00 到 53.00 μmol dm -3的线性范围内检测磺胺，检测限和定量限分别为 0.27 和 0.91 μmol dm -3， 分别。重要的是，该传感器显示出作为药物制剂和尿样中磺胺定量分析工具的实际应用。此处详述的电化学方法已使用分光光度法对药物样品进行了验证。所提出的方法具有良好的线性、低定量和检测限，在药物和尿液样品中具有出色的适用性。
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