Journal of Inorganic and Organometallic Polymers and Materials ( IF 3.9 ) Pub Date : 2021-08-07 , DOI: 10.1007/s10904-021-02082-2 Natarajan Muthusamy 1 , Paulkumar Kanniah 1 , Prasanthkumar Vijayakumar 1 , Umavanitha Murugan 1 , Divya Sunder Raj 1 , Umamaheswari Sankaran 1
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Abstract
The present work demonstrates the non-hazardous and environmentally benevolent green fabrication of silver nanoparticles using Terminalia chebula (T. chebula) seed extract. Characterization of silver nanoparticles was carried out by UV–Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction (XRD), atomic force microscopic (AFM) analysis, scanning electron microscopic (SEM) and transmission electron microscopic (TEM). The appearance of surface plasmon resonance peak at 440 UV–Vis spectra revealed the production of silver nanoparticles. The XRD analysis exhibited that the silver nanoparticles were crystalline. The SEM and AFM analysis represented that the synthesized silver nanoparticles were polydispersed with size ranges from 30 to 150 nm. TEM observations showed the production of differently shaped silver nanoparticles includes hexagonal and spherical. The LC–MS data revealed phenolic acids like ellagic acid, gallic acid, chebulic acid, and corilagin in the seed extract of T. chebula. The T. chebula seed extract fabricated silver nanoparticles displayed potent activity against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. Besides, the cytotoxic activity of the silver nanoparticles was examined against the McCoy fibroblast cell line. Herein, the viability of cells was highly inhibited at 100 µg/ml, and the IC50 value was obtained at 67.26 μg/ml. The apoptosis and nuclear fragmentation induction of silver nanoparticles was identified by DAPI staining assay.
Graphic Abstract
中文翻译：
银纳米粒子的绿色启发制造及其潜在的体外细胞毒性和抗菌活性
摘要
目前的工作证明了使用Terminalia chebula ( T . chebula) 种子提取物。通过紫外-可见光谱、傅里叶变换红外光谱、X 射线衍射 (XRD)、原子力显微镜 (AFM) 分析、扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 对银纳米粒子进行表征。440 UV-Vis 光谱处的表面等离子体共振峰的出现表明银纳米颗粒的产生。XRD分析表明银纳米颗粒是结晶的。SEM 和 AFM 分析表明合成的银纳米粒子是多分散的，尺寸范围为 30 到 150 nm。TEM 观察显示不同形状的银纳米粒子的产生包括六边形和球形。LC-MS 数据显示，植物种子提取物中的酚酸，如鞣花酸、没食子酸、chebulic 酸和 corilaginŤ。切布拉。该Ť。chebula种子提取物制造的银纳米颗粒显示出对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和幽门螺杆菌的有效活性。此外，还检测了银纳米颗粒对 McCoy 成纤维细胞系的细胞毒活性。在此，细胞活力在 100 μg/ml 时受到高度抑制，IC 50值在 67.26 μg/ml 时获得。通过DAPI染色测定鉴定银纳米颗粒的细胞凋亡和核碎裂诱导。
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