JBIC Journal of Biological Inorganic Chemistry ( IF 2.7 ) Pub Date : 2021-08-04 , DOI: 10.1007/s00775-021-01881-9 Daniela Escher 1 , M Nur Hossain 2 , Heinz-Bernhard Kraatz 2 , Jens Müller 1
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Films of four different DNA quadruplex-forming (G4) sequences (c-KIT, c-MYC, HTelo, and BCL2) on gold surfaces were investigated by electrochemical impedance spectroscopy (EIS) to evaluate whether they evoke unique electrochemical responses that can be used for their identification. This could render EIS an alternative means for the determination of G4 sequences of unknown structure. Towards, this end, cation-dependent topology changes in the presence of either K+, K+ in combination with Li+, or Pb2+ in the presence of Li+ were first evaluated by circular dichroism (CD) spectroscopy, and electrochemical studies were performed subsequently. As a result, G4-sequence specific charge transfer resistance (RCT) patterns were in fact observed for each G4 sequence, allowing their discrimination by EIS.
Graphic abstract
中文翻译：
DNA四链体序列的金属依赖性电化学鉴别
通过电化学阻抗谱 (EIS) 研究了金表面上四种不同 DNA 四链体形成 (G4) 序列 ( c-KIT、c-MYC、HTelo 和BCL2 ) 的薄膜，以评估它们是否会引起可用于的独特电化学响应以供他们识别。这可以使 EIS 成为确定未知结构的 G4 序列的替代方法。为此，在 K +、K +与 Li +的组合存在下，或在 Li +存在下Pb 2+存在时，依赖于阳离子的拓扑结构发生变化首先通过圆二色谱（CD）光谱进行评估，随后进行电化学研究。结果，实际上对于每个 G4 序列都观察到了 G4序列特定电荷转移电阻 ( R CT ) 模式，从而可以通过 EIS 对其进行区分。
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