Abstract

Screening of gut flora of the estuarine water fish ‘Chelon parsia’ for the presence of potential antibiotic producers resulted in finding a new strain Bacillus subtilis PP2. Further investigations focused on the active principle deduced that the molecule is a peptide class of antimicrobial agent with m/z of 1006.66. The culture condition optimization with improved combination and concentration of nitrogen and carbon sources had profound effect on bacterial growth as well as peptide production. In the preliminary stage of drug intervention, the partially characterized peptide antibiotic was found to be a broad-spectrum antifungal agent that can act against a number of fungal pathogens. The efficacy measurement indicated that the minimum inhibitory concentration (MIC) range of the peptide against various fungal pathogens ranges from 8.5 to 14.5 μg/mL. In terms of antibacterial activity, the molecule exhibits high efficacy, only in the narrow spectrum range. Antibacterial activity was observed against few bacterial pathogens with significantly pleasing MIC range (2.5 to 4.5 μg/mL) when compared with standard antibacterial drugs. The powerful surfactant property of the molecule opens up extended application into various fields. The novelty of the molecule is under investigation where the unique mass range (m/z 1006.66) of the molecule among the other peptide antibiotics produced by Bacillus subtilis is the key highlight. The findings of the present study suggest that the molecule can be proposed as a lead for the development of a multipurpose antimicrobial agent.

中文翻译：

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河口水鱼 'Chelon parsia' 肠道共生细菌的抗菌作用

摘要

筛选河口水鱼' Chelon parsia '的肠道菌群中是否存在潜在的抗生素生产者，结果发现了一种新菌株枯草芽孢杆菌PP2。进一步研究集中在活性成分上推断出该分子是具有m / z的肽类抗菌剂1006.66。通过改进氮源和碳源的组合和浓度来优化培养条件对细菌生长和肽生产具有深远的影响。在药物干预的初期，部分表征的肽抗生素被发现是一种广谱抗真菌剂，可以对抗多种真菌病原体。功效测量表明肽对各种真菌病原体的最小抑制浓度 (MIC) 范围为 8.5 至 14.5 μg/mL。在抗菌活性方面，该分子仅在窄谱范围内表现出高效能。与标准抗菌药物相比，观察到对少数细菌病原体具有显着令人满意的 MIC 范围（2.5 至 4.5 μg/mL）的抗菌活性。该分子强大的表面活性剂特性开辟了在各个领域的扩展应用。该分子的新颖性正在研究中，其中独特的质量范围（m / z 1006.66) 的分子在枯草芽孢杆菌产生的其他肽抗生素中是关键亮点。本研究的结果表明，该分子可以作为开发多用途抗菌剂的先导物。