Abstract

Diversity of methano- and methylotrophic bacteria in enrichments from the oxidized sediment layer maintained at 10°C in mineral media with nitrogen compounds (\({\text{NO}}_{3}^{ - }\) and \({\text{NH}}_{4}^{ + }\)) and methanol was studied using high-throughput sequencing of the V2–V3 region of the 16S rRNA gene. Methanotrophs of the bottom sediment belonged to the order Methylococcales, with predominance of the genus Methylobacter (6.4 and 4.6% of the total number of sequences, respectively). Members of the methylotrophic community belonged to the genus Methylotenera (family Methylophilaceae). Enrichments in the medium with ammonium and methane were characterized by the highest diversity of methanotrophs, containing 22 OTUs of the family Methylococcaceae, nine of which were not detected under other cultivation conditions. Altogether, only six out of 28 OTUs of methanotrophic bacteria exhibited similarity to cultured members of Methylobacter, Metyloglobulus, and Methylomicrobium, while others showed different levels of similarity (94–99%) to uncultured members of the family Methylococcaceae. The presence of 0.01% methanol promoted preferential development of methylotrophic bacteria, while 0.5% methanol suppressed the growth of methanotrophic bacteria irrespective of the nitrogen source added.

中文翻译：

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甲醇和矿物氮化合物对贝加尔湖甲烷渗流沉积物中甲烷营养富集物组成的影响

摘要

在含氮化合物 ( \({\text{NO}}_{3}^{ - }\)和\({\ text{NH}}_{4}^{ + }\) ) 和甲醇使用 16S rRNA 基因的 V2-V3 区域的高通量测序进行了研究。底部沉积物的甲烷营养属于顺序Methylococcales，与属的优势Methylobacter（6.4和4.6分别序列的总数的％）。在甲基营养社区的成员属于属Methylotenera（家庭Methylophilaceae）。由甲烷氧化的最高分集与铵和甲烷的富集介质进行了表征，含有22个OTU家庭Methylococcaceae，其中九个其它培养条件下未检测到。总的来说，在 28 个甲烷营养细菌的 OTU 中，只有 6 个与甲基杆菌、甲基球菌和甲基微生物菌的培养成员表现出相似性，而其他人则表现出与甲基球菌科未培养成员的不同程度的相似性（94-99%）。0.01% 甲醇的存在促进了甲基营养菌的优先发育，而 0.5% 甲醇抑制了甲烷营养菌的生长，而与添加的氮源无关。