A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With rhodamine 6G as the background fluorescent substance, neomycin with weak ultraviolet absorption can be detected effectively. The types and concentrations of background buffer, pH, and separation voltage which affected the separation and analysis were all studied. With the optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%.

中文翻译：

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毛细管电泳-间接激光诱导荧光检测鱼中的新霉素

建立了一种毛细管电泳-间接激光诱导荧光检测方法，用于鱼类新霉素的检测。以罗丹明6G为背景荧光物质，可有效检测紫外吸收较弱的新霉素。研究了影响分离分析的背景缓冲液的类型和浓度、pH值和分离电压。在最佳条件下，新霉素的检出限为5.0 ng/g，鱼样品的回收率在95.2-99.7%之间，相对标准偏差在2.7-3.6%之间。