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Capillary Electrophoresis-Indirect Laser-Induced Fluorescence Detection of Neomycin in Fish

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Abstract

A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With rhodamine 6G as the background fluorescent substance, neomycin with weak ultraviolet absorption can be detected effectively. The types and concentrations of background buffer, pH, and separation voltage which affected the separation and analysis were all studied. With the optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%.

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Acknowledgements

The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (NSFC, Grant No. 21005021), Natural Science Foundation of Guangdong Province (No. 2016A030313740, No. 2021A1515011410) and Guangdong Provincial Science and Technology Project (No. 2016B030303002).

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Authors and Affiliations

  1. College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Yejing Huang, Xiufen Han, Xiao Yu, Shumei Wang & Haiyun Zhai

  2. Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Shumei Wang

  3. Engineering and Technology Research Center for Chinese Materia Medica Quality of Guangdong Province, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Shumei Wang

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  1. Yejing Huang
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  2. Xiufen Han
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  3. Xiao Yu
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Correspondence to Xiao Yu or Haiyun Zhai.

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Huang, Y., Han, X., Yu, X. et al. Capillary Electrophoresis-Indirect Laser-Induced Fluorescence Detection of Neomycin in Fish. Chromatographia 84, 861–868 (2021). https://doi.org/10.1007/s10337-021-04075-2

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