The study of microorganisms in aquaponics is an important topic which requires more research before exploiting the full potential of beneficial microorganisms. In this experiment, we focused on the evolution over time of the bacterial communities in four compartments of an aquaponic system i.e., the sump, the biofilter, the lettuce rhizoplane and lettuce root. We studied these communities over the course of a lettuce growth cycle via regular sampling and sequencing of the 16S rRNA gene of the collected bacteria. We also followed the physicochemical parameters of the aquaponic water throughout the experiment. Results show that a different community could be found in each compartment and that all four communities were stable throughout time and resilient to naturally occurring water parameter changes which characterize functioning aquaponic systems. Furthermore, the communities of the sump and biofilter also seem stable over the years as the predominant taxa (Luteolibacter, Flavobacterium, Nitrospira) observed in our study are similar to the ones previously reported for this aquaponic system. Finally, our results provide proof for similarities between aquaponic and soil borne lettuce root communities (gammaproteobacteria, Flavobacterium, Pseudomonadaceae, Sphingomonadaceae) thus showing that aquaponics can be similar to soil production in terms of microbial life.

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生菜生长周期中鱼菜共生菌群的生态学研究

鱼菜共生中微生物的研究是一个重要的课题，在充分发挥有益微生物的潜力之前需要更多的研究。在这个实验中，我们专注于鱼菜共生系统的四个隔室中细菌群落随时间的演变，即水槽、生物过滤器、生菜根状体和生菜根。我们通过对收集到的细菌的 16S rRNA 基因进行定期采样和测序，在生菜生长周期的过程中研究了这些群落。在整个实验过程中，我们还跟踪了鱼菜共生水的理化参数。结果表明，在每个隔间中都可以找到不同的群落，并且所有四个群落在整个时间段内都是稳定的，并且对自然发生的水参数变化具有弹性，这些变化是功能性鱼菜共生系统的特征。在我们的研究中观察到的Luteolibacter、Flavobacterium、Nitrospira）与之前报道的这种鱼菜共生系统相似。最后，我们的结果为鱼菜共生和土生莴苣根群落（γ-变形菌、黄杆菌、假单胞菌科、鞘氨醇单胞菌科）之间的相似性提供了证据，从而表明鱼菜共生在微生物生命方面与土壤生产相似。