The search for new compounds with activity against Paracoccidioides, etiologic agents of Paracoccidioidomycosis (PCM), is extremely necessary due to the current scenario of the available therapeutic arsenal. Treatment is restricted to three classes of antifungals with side effects. Curcumin is a polyphenol with antifungal effects that is extracted from Curcuma longa. The present work aimed to evaluate the activity of curcumin in different species of Paracoccidioides and to evaluate the potential molecular targets of curcumin using computational strategies. In addition, interactions with classic antifungals used in the treatment of PCM were evaluated. Curcumin inhibits the growth of Paracoccidioides spp. exerting a fungicidal effect. The combination of curcumin with amphotericin B, co-trimoxazole, and itraconazole showed a synergistic or additive interaction. Molecular targets as superoxide dismutase, catalase, and isocitrate lyase were proposed based on in silico approaches. Curcumin affects the fungal plasma membrane and increases the production of reactive oxygen species. Therefore, curcumin is a good alternative for the treatment of PCM.

鉴于目前可用的治疗手段，寻找具有对抗副球孢子菌病 (PCM) 病原菌副球孢子菌活性的新化合物是极其必要的。治疗仅限于三类具有副作用的抗真菌药物。姜黄素是从姜黄中提取的具有抗真菌作用的多酚。目前的工作旨在评估姜黄素在不同副球孢子菌物种中的活性，并使用计算策略评估姜黄素的潜在分子靶标。此外，还评估了与治疗 PCM 时使用的经典抗真菌药物的相互作用。姜黄素抑制副球孢子菌属的生长。发挥杀菌作用。姜黄素与两性霉素 B、复方新诺明和伊曲康唑的组合显示出协同或相加的相互作用。基于计算机方法提出了超氧化物歧化酶、过氧化氢酶和异柠檬酸裂解酶等分子靶标。姜黄素影响真菌质膜并增加活性氧的产生。因此，姜黄素是治疗PCM的良好替代品。