Colloid and Polymer Science ( IF 2.2 ) Pub Date : 2021-07-29 , DOI: 10.1007/s00396-021-04871-0 Muhammad Yasir Siddique 1 , Iqra Alamgir 1 , Sajjad Hussain Sumrra 1 , Muhammad Ashfaq 1 , Muhammad Faizan Nazar 2 , Muhammad Safdar 3 , Salah Ud-Din Khan 4 , Omar Saleh Al-Zaid 4 , Ashfaq Ahmad 5 , Hassan M. Al Swaidan 5 , Rawaiz Khan 6
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In this study, an oil-in-water microemulsion (µE) formulation was developed using clove oil/Brij-35/isopropanol/water to solubilize fluoroquinolone (FLQ) antibiotics, namely, Ciprofloxacin (CF), Levofloxacin (LF), and Moxifloxacin (MF). Through the mapping of pseudo-ternary phase diagram, optimum µE containing clove oil (18%) and water (26%) were established, maintaining the Brij-35/isopropanol ratio (1:1) to upheld the appropriate amount of FLQ, i.e., CF (3.8 wt. %), LF (5.2 wt. %), and MF (4.2 wt. %). Through optical microscopy and electrical conductivity, the structural transformation of as-formulated µE was analyzed. The peak-to-peak correlation of the FTIR study shows that FLQ have good compatibility with µE excipients, while the DLS results show monomodal size distribution of microdroplets. Similarly, FLQ fluorescence detection perceives the interface environment of the colloidal domain. In addition, the agar well diffusion method was used to evaluate the antibacterial potential of the formulated FLQ-loaded µEs, indicating the enhanced antibacterial activity against all bacterial strains.
Graphical abstract
中文翻译：
基于 Brij-35 的氟喹诺酮类抗生素微乳液的结构和探测动力学
在本研究中，使用丁香油/Brij-35/异丙醇/水开发了一种水包油微乳剂 (µE) 配方，以溶解氟喹诺酮 (FLQ) 抗生素，即环丙沙星 (CF)、左氧氟沙星 (LF) 和莫西沙星(MF)。通过拟三元相图的映射，确定了含有丁香油（18%）和水（26%）的最佳μE，保持Brij-35/异丙醇比例（1:1）以维持适当的FLQ量，即、CF(3.8wt.%)、LF(5.2wt.%)和MF(4.2wt.%)。通过光学显微镜和电导率，分析了配制的 µE 的结构转变。FTIR 研究的峰峰值相关性表明 FLQ 与 µE 赋形剂具有良好的相容性，而 DLS 结果显示微滴的单峰尺寸分布。相似地，FLQ荧光检测感知胶体域的界面环境。此外，琼脂井扩散法用于评估配制的 FLQ 负载 μE 的抗菌潜力，表明对所有细菌菌株的抗菌活性增强。
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