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Structural and probing dynamics of Brij-35-based microemulsion for fluoroquinolone antibiotics

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Abstract

In this study, an oil-in-water microemulsion (µE) formulation was developed using clove oil/Brij-35/isopropanol/water to solubilize fluoroquinolone (FLQ) antibiotics, namely, Ciprofloxacin (CF), Levofloxacin (LF), and Moxifloxacin (MF). Through the mapping of pseudo-ternary phase diagram, optimum µE containing clove oil (18%) and water (26%) were established, maintaining the Brij-35/isopropanol ratio (1:1) to upheld the appropriate amount of FLQ, i.e., CF (3.8 wt. %), LF (5.2 wt. %), and MF (4.2 wt. %). Through optical microscopy and electrical conductivity, the structural transformation of as-formulated µE was analyzed. The peak-to-peak correlation of the FTIR study shows that FLQ have good compatibility with µE excipients, while the DLS results show monomodal size distribution of microdroplets. Similarly, FLQ fluorescence detection perceives the interface environment of the colloidal domain. In addition, the agar well diffusion method was used to evaluate the antibacterial potential of the formulated FLQ-loaded µEs, indicating the enhanced antibacterial activity against all bacterial strains.

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Acknowledgements

The authors express their gratitude to the Department of Chemistry, University of Gujrat Pakistan, for providing laboratory facilities.

Funding

M.F. Nazar received financial support from Higher Education Commission of Pakistan through NRPU Project. 20–4557/NRPU/R&D/HEC/14/481. The authors also received financial support from Deanship of Scientific Research at King Saud University through research group no. RG-1441–384.

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Authors and Affiliations

  1. Department of Chemistry, University of Gujrat, Gujrat, 50700, Pakistan

    Muhammad Yasir Siddique, Iqra Alamgir, Sajjad Hussain Sumrra & Muhammad Ashfaq

  2. Department of Chemistry, University of Education Lahore, Multan Campus, 60700, Pakistan

    Muhammad Faizan Nazar

  3. Department of Pharmaceutics, Faculty of Pharmacy, Gomal University, Dera Ismail Khan, KPK, Pakistan

    Muhammad Safdar

  4. College of Engineering, Sustainable Energy Technologies Center, King Saud University, PO-Box 800, Riyadh, 11421, Saudi Arabia

    Salah Ud-Din Khan & Omar Saleh Al-Zaid

  5. Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Ashfaq Ahmad & Hassan M. Al Swaidan

  6. College of Dentistry, Engineer Abdullah Bugshan Research Chair for Dental and Oral Rehabilitation, King Saud University, Riyadh, 11545, Saudi Arabia

    Rawaiz Khan

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  1. Muhammad Yasir Siddique
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Siddique, M.Y., Alamgir, I., Nazar, M.F. et al. Structural and probing dynamics of Brij-35-based microemulsion for fluoroquinolone antibiotics. Colloid Polym Sci 299, 1479–1488 (2021). https://doi.org/10.1007/s00396-021-04871-0

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