当前位置: X-MOL 学术Annu. Rev. Anal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Analytical Technologies for Liquid Biopsy of Subcellular Materials
Annual Review of Analytical Chemistry ( IF 5.9 ) Pub Date : 2021-07-27 , DOI: 10.1146/annurev-anchem-091520-093931
Camila D.M. Campos 1, 2 , Katie Childers 3, 4 , Sachindra S.T. Gamage 4, 5 , Harshani Wijerathne 6 , Zheng Zhao 3, 4 , Steven A. Soper 3, 4, 5, 7, 8
Affiliation  

Liquid biopsy markers, which can be secured from a simple blood draw or other biological samples, are used to manage a variety of diseases and even monitor for bacterial or viral infections. Although there are several different types of liquid biopsy markers, the subcellular ones, including cell-free DNA, microRNA, extracellular vesicles, and viral particles, are evolving in terms of their utility. A challenge with liquid biopsy markers is that they must be enriched from the biological sample prior to analysis because they are a vast minority in a mixed population, and potential interferences may be present in the sample matrix that can inhibit profiling the molecular cargo from the subcellular marker. In this article, we discuss existing and developing analytical enrichment platforms used to isolate subcellular liquid biopsy markers, and discuss their figures of merit such as recovery, throughput, and purity.

中文翻译:


亚细胞材料液体活检的分析技术

液体活检标记物可以从简单的抽血或其他生物样本中获取,用于管理各种疾病,甚至监测细菌或病毒感染。尽管有几种不同类型的液体活检标记物,但亚细胞标记物,包括无细胞 DNA、微 RNA、细胞外囊泡和病毒颗粒,在其效用方面正在不断发展。液体活检标志物的一个挑战是,它们必须在分析前从生物样品中富集,因为它们是混合群体中的极少数,并且样品基质中可能存在潜在干扰,这些干扰可能会抑制从亚细胞分析分子货物标记。在本文中,我们讨论了用于分离亚细胞液体活检标志物的现有和正在开发的分析富集平台,

更新日期:2021-07-28
down
wechat
bug