Folia Microbiologica ( IF 2.4 ) Pub Date : 2021-07-27 , DOI: 10.1007/s12223-021-00900-1 María Belén Abdulhamid 1 , Luciana Costas 1 , Flavia Del Valle Loto 1 , Mario Domingo Baigorí 1, 2 , Licia María Pera 1
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The successfulness of a lipase-catalyzed industrial process depends on a proper lipase selection. In this work, an alternative screening platform for industrially important biotransformations catalyzed by microbial lipases was proposed. Thus, the reactivity of sixty lipase activities from spore-forming microorganisms towards hydrolytic and transesterification reactions by using p-nitrophenyl palmitate as a chromogenic acyl donor substrate was explored. Only three biocatalysts were capable of catalyzing all reactions tested. Fourteen biocatalysts did not show hydrolytic activity at all; however, they displayed transesterification activities using ethanol, starch, low-methoxyl (LM) pectin, high-methoxyl (HM) pectin, or vitamin C as acyl acceptors. Using heat-treated biocatalysts, hydrolytic activities were not highly correlated with the corresponding transesterification activities using ethanol (r = −0.058, p = 0.660), starch (r = 0.431, p = 0.001), LM pectin (r = −0.010, p = 0.938), HM pectin (r = 0.167, p = 0.202), and vitamin C (r = −0.048, p = 0.716) as acyl acceptor. In addition, to the best of our knowledge, several transesterification activities produced from microorganisms of the genus Bacillus, Brevibacillus, Lysinibacillus, Geobacillus, or Sporosarcina were reported for first time. Finally, the global lipase market was presented and segmented by date, application, geography and player highlighting the commercial contribution of microbial lipases.
Graphical abstract
中文翻译：
微生物脂肪酶催化的工业生物转化：筛选平台和商业方面
脂肪酶催化工业过程的成功取决于适当的脂肪酶选择。在这项工作中，提出了一种替代筛选平台，用于由微生物脂肪酶催化的工业上重要的生物转化。因此，产孢微生物的 60 种脂肪酶活性对水解和酯交换反应的反应性通过使用p-探索了作为显色酰基供体底物的硝基苯基棕榈酸酯。只有三种生物催化剂能够催化所有测试的反应。十四种生物催化剂根本没有显示水解活性；然而，他们使用乙醇、淀粉、低甲氧基 (LM) 果胶、高甲氧基 (HM) 果胶或维生素 C 作为酰基受体显示出酯交换活性。使用经过热处理的生物催化剂，水解活性与使用乙醇 ( r = -0.058, p = 0.660)、淀粉 ( r = 0.431, p = 0.001)、LM 果胶 ( r = -0.010, p = 0.938), HM 果胶 ( r = 0.167, p = 0.202) 和维生素 C ( r = -0.048, p = 0.716) 作为酰基受体。此外，据我们所知，一些酯交换的活动自属的微生物产生的芽孢杆菌，短短芽孢，Lysinibacillus，芽孢杆菌属，或Sporosarcina报告了第一次。最后，介绍了全球脂肪酶市场并按日期、应用、地理和参与者进行了细分，突出了微生物脂肪酶的商业贡献。
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