The study was aimed to determine the effects of foliar applications of thidiazuron and putrescine, two compounds that may cause iron (Fe) remobilization, in pot marigold (Calendula officinalis L.) grown in controlled conditions under a limited Fe supply. In a first experiment, plants were grown in a greenhouse in a mixture of sand and perlite with pre-growth/growth Fe concentrations ranging from 0 to 20 µM, and treated three consecutive times with foliar sprays of 0 or 45.4 µM thidiazuron. In a second experiment, plants were grown in a greenhouse in hydroponics with pre-growth/growth Fe concentrations in the nutrient solution ranging from 0 to 20 µM, and treated three consecutive times with foliar sprays of 0, 2.27, or 5.67 mM putrescine. Parameters measured included leaf photosynthetic pigments and Fe concentrations, root ferric chelate reductase activities, photosynthesis rates and peroxidase in leaf extracts in the first experiment, and leaf photosynthetic pigments, leaf and root micronutrient concentrations, root ferric chelate reductase activities, and superoxide dismutase and peroxidase activities in leaf extracts in the second experiment. Results indicate that foliar thidiazuron and putrescine treatments in the µM and mM ranges, respectively, improve Fe transport to the leaf under zero or low supply of Fe. This indicates that foliar treatments with thidiazuron and putrescine increase remobilization of pre-existing plant Fe pools. This could be an additional tool for the optimization of Fe nutrition in ornamental plants such as C. officinalis when grown in controlled conditions.

该研究旨在确定在盆栽万寿菊（Calendula officinalis）中叶面喷施噻唑脲和腐胺这两种可能导致铁 (Fe) 再动员的化合物的影响L.) 在有限的 Fe 供应下在受控条件下生长。在第一个实验中，植物在沙子和珍珠岩混合物的温室中生长，生长前/生长的 Fe 浓度范围为 0 到 20 µM，并用 0 或 45.4 µM 噻二唑隆叶面喷洒连续 3 次处理。在第二个实验中，植物在水培温室中生长，营养液中生长前/生长的 Fe 浓度范围为 0 到 20 µM，并用 0、2.27 或 5.67 mM 腐胺叶面喷洒连续 3 次处理。测量的参数包括叶片光合色素和 Fe 浓度、根铁螯合还原酶活性、光合作用速率和第一个实验中叶片提取物中的过氧化物酶，以及叶片光合色素、叶片和根微量营养素浓度、在第二个实验中，叶提取物中的根铁螯合还原酶活性以及超氧化物歧化酶和过氧化物酶活性。结果表明，分别在 μM 和 mM 范围内的叶面噻唑脲和腐胺处理可在零或低 Fe 供应下改善 Fe 向叶片的转运。这表明用噻唑脲和腐胺进行叶面处理增加了预先存在的植物铁库的再动员。这可能是优化观赏植物中铁营养的额外工具，例如 这表明用噻唑脲和腐胺进行叶面处理增加了预先存在的植物铁库的再动员。这可能是优化观赏植物中铁营养的额外工具，例如 这表明用噻唑脲和腐胺进行叶面处理增加了预先存在的植物铁库的再动员。这可能是优化观赏植物中铁营养的额外工具，例如C. officinalis在受控条件下生长。