The acute toxicity and influence on the immune system of the melanin–polysaccharide complex and β-glucans isolated from horse chestnut shells (Aesculus hippocastanum L.) were studied. The β-glucans were obtained from the melanin–polysaccharide complex by separation and purification. The results indicated that the studied compounds were Class VI relatively harmless substances with an LD₅₀ of >6,000 mg/kg. Results of the immunotropic activity testing showed that intragastric administration to animals of the polysaccharide complex and β-glucans at doses of 5 and 0.5 mg/kg stimulated humoral immunity. The numbers of antibody-producing cells per million spleen cells increased statistically significantly by 2.46 and 2.74 times, respectively. The test compounds also affected cellular immunity by increasing the number of nucleus-containing cells in the thymus, spleen, lymph nodes, and macrophages in peritoneal fluid.

研究了从七叶树壳（Aesculus hippocastanum L.）中分离的黑色素-多糖复合物和β-葡聚糖的急性毒性和对免疫系统的影响。β-葡聚糖是通过分离和纯化从黑色素-多糖复合物中获得的。结果表明，研究的化合物是 VI 类相对无害的物质，LD ₅₀>6,000 毫克/公斤。免疫活性测试的结果表明，将多糖复合物和 β-葡聚糖以 5 和 0.5 毫克/千克的剂量对动物进行胃内给药可刺激体液免疫。每百万个脾细胞中产生抗体的细胞数分别显着增加了 2.46 和 2.74 倍。测试化合物还通过增加胸腺、脾脏、淋巴结和腹腔液中的巨噬细胞中含核细胞的数量来影响细胞免疫。