The acute toxicity and influence on the immune system of the melanin–polysaccharide complex and β-glucans isolated from horse chestnut shells (Aesculus hippocastanum L.) were studied. The β-glucans were obtained from the melanin–polysaccharide complex by separation and purification. The results indicated that the studied compounds were Class VI relatively harmless substances with an LD50 of >6,000 mg/kg. Results of the immunotropic activity testing showed that intragastric administration to animals of the polysaccharide complex and β-glucans at doses of 5 and 0.5 mg/kg stimulated humoral immunity. The numbers of antibody-producing cells per million spleen cells increased statistically significantly by 2.46 and 2.74 times, respectively. The test compounds also affected cellular immunity by increasing the number of nucleus-containing cells in the thymus, spleen, lymph nodes, and macrophages in peritoneal fluid.
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 55, No. 4, pp. 44 – 48, April, 2021.
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Filatova, A.V., Turaev, A.S., Vypova, N.L. et al. Isolation of Polysaccharides from Horse Chestnut (Aesculus hippocastanum L.) Shells and their Acute Toxicity and Influence on the Immune System. Pharm Chem J 55, 373–377 (2021). https://doi.org/10.1007/s11094-021-02430-z
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DOI: https://doi.org/10.1007/s11094-021-02430-z