Skip to main content
Log in

Isolation of Polysaccharides from Horse Chestnut (Aesculus hippocastanum L.) Shells and their Acute Toxicity and Influence on the Immune System

  • MEDICINAL PLANTS
  • Published:
Pharmaceutical Chemistry Journal Aims and scope

The acute toxicity and influence on the immune system of the melanin–polysaccharide complex and β-glucans isolated from horse chestnut shells (Aesculus hippocastanum L.) were studied. The β-glucans were obtained from the melanin–polysaccharide complex by separation and purification. The results indicated that the studied compounds were Class VI relatively harmless substances with an LD50 of >6,000 mg/kg. Results of the immunotropic activity testing showed that intragastric administration to animals of the polysaccharide complex and β-glucans at doses of 5 and 0.5 mg/kg stimulated humoral immunity. The numbers of antibody-producing cells per million spleen cells increased statistically significantly by 2.46 and 2.74 times, respectively. The test compounds also affected cellular immunity by increasing the number of nucleus-containing cells in the thymus, spleen, lymph nodes, and macrophages in peritoneal fluid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

Similar content being viewed by others

References

  1. R. Chang, J. Altern. Complement. Med., No. 8, 559 – 565 (2002); doi: 10.1089 / 107555302320825066.

  2. V. E. C. Ooi and F. Liu, Curr. Med. Chem., No. 7, 715 – 729 (2000); doi: 10.2174 / 0929867003374705.

  3. R. Seijelid, J. Bogwald, and A. Lundwall, Exp. Cell Res., 131(1), 121 – 129 (1981).

    Article  CAS  Google Scholar 

  4. S.-H. Young, J. Ye, D. G. Frazer, et al., J. Biol. Chem., 276(23), 20781 – 20787 (2001); doi: 10.1074 / jbc. M101111200.

  5. N. N. Besednova, L. A. Ivanushko, T. N. Zvyagintseva, et al., Antibiot. Khimioter., No. 2, 37 – 44 (2000).

  6. D. R. Dixon and R. P. Darveau, J. Dent. Res., 84(7), 584 – 595 (2005); doi: 10.1177 / 154405910508400702.

  7. S. L. Gaffin, Prog. Clin. Biol. Res., 29, 221 – 227 (1979); doi: 10.1089 / 107555302320825066.

  8. V. A. Soboleva and A. A. Kolomiets, Provizor, No. 21, 45 – 49 (2007).

  9. I. Ya. Zakharova and L. V. Kosenko, Methods for Studying Microbial Polysaccharides [in Russian], Kiev (1982).

  10. M. Dubois, K. A. Gilles, et al., Anal. Chem., 28, 350 – 356 (1956); doi.org / 10.1021 / ac60111a017.

  11. A. M. Staub, “Removal of protein – Sevag method,” Methods Carbohydr. Chem., 5, 5 – 6 (1965).

    CAS  Google Scholar 

  12. GOST 32373-2013. Testing of Chemicals of Health Hazard – Test for Dermal Toxicity [in Russian], Standartinform, Moscow (2019).

  13. A. N. Mironov, Handbook for Preclinical Drug Trials [in Russian], Part 1, Grif i K, Moscow (2012), pp. 334 – 337.

  14. A. N. Mironov (ed.), Methodical Recommendations for Preclinical Study of Immunotropic Activity of Drugs [in Russian], Moscow (2012), pp. 624 – 640.

  15. N. K. Jerne and A. A. Nordin, “Plaque formation in agar by single antibody-producing cells,” Science, 140(3565), 405 (1963).

  16. O. Yu. Rebrova, Statistical Analysis of Medical Data. Applied Program Package [in Russian], Media Sfera, Moscow (2003).

  17. A. E. Platonov, Statistical Data Analysis in Medicine and Biology [in Russian], Izd. RAMN, Moscow (2000).

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. V. Filatova.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 55, No. 4, pp. 44 – 48, April, 2021.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Filatova, A.V., Turaev, A.S., Vypova, N.L. et al. Isolation of Polysaccharides from Horse Chestnut (Aesculus hippocastanum L.) Shells and their Acute Toxicity and Influence on the Immune System. Pharm Chem J 55, 373–377 (2021). https://doi.org/10.1007/s11094-021-02430-z

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-021-02430-z

Keywords

Navigation