Intracellular free Ca²⁺ ([Ca²⁺]_i) dysregulation occurs in coronary smooth muscle (CSM) in atherosclerotic coronary artery disease (CAD) of metabolic syndrome (MetS) swine. Our goal was to determine how CAD severity, arterial structure, and MetS risk factors associate with [Ca²⁺]_i dysregulation in human CAD compared to changes in Ossabaw miniature swine. CSM cells were dispersed from coronary arteries of explanted hearts from transplant recipients and from lean and MetS swine with CAD. CSM [Ca²⁺]_i elicited by Ca²⁺ influx and sarcoplasmic reticulum (SR) Ca²⁺ release and sequestration was measured with fura-2. Increased [Ca²⁺]_i signaling was associated with advanced age and a greater media area in human CAD. Decreased [Ca²⁺]_i signaling was associated with a greater number of risk factors and a higher plaque burden in human and swine CAD. Similar [Ca²⁺]_i dysregulation exhibited in human and Ossabaw swine CSM provides strong evidence for the translational relevance of this large animal model.

代谢综合征（MetS）猪的动脉粥样硬化性冠状动脉疾病（CAD）中，细胞内游离Ca ²⁺ ([Ca ²⁺ ] _{i ) 失调发生在冠状动脉平滑肌(CSM) 中。}我们的目标是确定与Ossabaw 小型猪的变化相比，CAD 严重程度、动脉结构和 MetS 危险因素与人类 CAD 中[Ca ²⁺ ] _{i失调的关系。}CSM 细胞分散自移植受者以及患有 CAD 的瘦肉猪和 MetS 猪的移植心脏的冠状动脉。^{由Ca 2+}流入引起的CSM [Ca ²⁺ ] _i和肌浆网(SR) Ca ²⁺释放和封存用fura-2 测量。[Ca ²⁺ ] _i信号传导增加与高龄和人类 CAD 中更大的介质区域相关。[Ca ²⁺ ] _i信号传导减少与人类和猪 CAD 中更多的危险因素和更高的斑块负荷相关。人类和 Ossabaw 猪 CSM 中表现出的类似 [Ca ²⁺ ] _i失调为这种大型动物模型的转化相关性提供了强有力的证据。