In the study, fluorescent imaging of live cells was performed using fluorescent carbon quantum dots derived from edible mushrooms species; Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus as a fluorophore agent. Carbon quantum dots were synthesized through a facile and low-cost method based on microwave irradiation of dried mushroom samples in hydrogen peroxide solution under optimized conditions (microwave energy, solution type, duration of microwave treatment, amount of mushroom). Upon purification with centrifugation, microfiltration, and dialysis, the lyophilized carbon quantum dots were identified through UV–visible, fluorescence and FT-IR, X-ray photoelectron spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, and quantum yield calculation. Cell viability assessment of the carbon quantum dots was evaluated against human epithelial cell line PNT1A using the Alamar Blue Assay. In vitro fluorescence cell imaging studies demonstrated that the carbon dots could dynamically penetrate the cell membrane and nuclear membrane and localize in both the cytoplasm and the nucleus.

在这项研究中，使用源自食用蘑菇物种的荧光碳量子点对活细胞进行荧光成像；双孢蘑菇、平菇和牛肝菌作为荧光团试剂。在优化条件（微波能量、溶液类型、微波处理时间、蘑菇数量）下，通过一种简便且低成本的方法合成了碳量子点，该方法基于在过氧化氢溶液中微波照射干燥蘑菇样品。经过离心、微滤和透析纯化后，通过紫外-可见光、荧光和 FT-IR、X 射线光电子能谱、X 射线衍射、高分辨率透射电子显微镜和量子产率计算来鉴定冻干碳量子点。使用 Alamar Blue Assay 对照人上皮细胞系 PNT1A 评估碳量子点的细胞活力。体外荧光细胞成像研究表明，碳点可以动态穿透细胞膜和核膜，定位于细胞质和细胞核中。