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Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus

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Abstract

In the study, fluorescent imaging of live cells was performed using fluorescent carbon quantum dots derived from edible mushrooms species; Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus as a fluorophore agent. Carbon quantum dots were synthesized through a facile and low-cost method based on microwave irradiation of dried mushroom samples in hydrogen peroxide solution under optimized conditions (microwave energy, solution type, duration of microwave treatment, amount of mushroom). Upon purification with centrifugation, microfiltration, and dialysis, the lyophilized carbon quantum dots were identified through UV–visible, fluorescence and FT-IR, X-ray photoelectron spectroscopy, X-ray diffraction, high-resolution transmission electron microscopy, and quantum yield calculation. Cell viability assessment of the carbon quantum dots was evaluated against human epithelial cell line PNT1A using the Alamar Blue Assay. In vitro fluorescence cell imaging studies demonstrated that the carbon dots could dynamically penetrate the cell membrane and nuclear membrane and localize in both the cytoplasm and the nucleus.

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Funding

This project study was funded by Selcuk University Research Foundation (project number: BAP-19401118).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Selcuk University, 42075, Konya, Turkey

    Idris Sargin, Serdar Karakurt & Gulsin Arslan

  2. Cumra Colloge of Applied Sciences, Selcuk University, 42500, Cumra, Konya, Turkey

    Sinan Alkan

Authors
  1. Idris Sargin
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  2. Serdar Karakurt
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  3. Sinan Alkan
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  4. Gulsin Arslan
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Contributions

Conceptualization: [Gulsin Arslan, Idris Sargin, Sedar Karakurt]; Design: [Gulsin Arslan, Idris Sargin, Sedar Karakurt]; Material preparation: [Gulsin Arslan, Idris Sargin, Sinan Alkan]; Data collection and analysis: [Gulsin Arslan, Idris Sargin, Serdar Karakurt]; Writing-original draft: [Gulsin Arslan, Idris Sargin, Serdar Karakurt]; Mushrooms’species identification: [Sinan Alkan].

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Correspondence to Idris Sargin.

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Sargin, I., Karakurt, S., Alkan, S. et al. Live Cell Imaging With Biocompatible Fluorescent Carbon Quantum Dots Derived From Edible Mushrooms Agaricus bisporus, Pleurotus ostreatus, and Suillus luteus. J Fluoresc 31, 1461–1473 (2021). https://doi.org/10.1007/s10895-021-02784-3

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  • DOI: https://doi.org/10.1007/s10895-021-02784-3

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