A Gram-stain-positive, non-pigmented, coccus-shaped, facultatively anaerobic and α-hemolytic bacterium designated as INB8^T was isolated from a hypersaline marine water sample collected at the Inland Sea of Qatar. The isolate was able to grow at 25–40 °C (optimum, 30 °C), at pH 5–11 and with 2–8% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain INB8^T was placed within the family Aerococcaceae with the highest sequence similarity to Facklamia tabacinasalis CCUG 30090^T (99.5%), followed by Facklamia hominis CCUG 36813^T (93.9%), Facklamia sourekii Y17312^T (93.8%), Facklamia ignava CCUG 37419^T (93.6%), Facklamia miroungae CCUG 42728^T (93.5%), Suicoccus acidiformans ZY16052^T (93.5%), Facklamia languida CCUG 37842^T (93.2%), Ignavigranum ruoffiae (93.1%), and Dolosicoccus paucivorans DSM 15742^T (90.8%). Average nucleotide identity and digital DNA–DNA hybridization values between strain INB8^T and F. tabacinasalis CCUG 30090^T were determined to be 94.5% and 58.9% respectively, confirming strain INB8^T represents a novel species. The major fatty acids were C_14:0, C_16:0, C_18:0 and C_18:1 ω9c_. The G + C content of strain INB8^T determined from the genome was 36.3 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic information, it is proposed that Facklamia tabacinasalis should be reclassified as Ruoffia tabacinasalis, Facklamia ignava be reclassified as Falseniella ignava, and Facklamia sourekii be reclassified Hutsoniella sourekii. It is further proposed that strain INB8^T should be classified as a species of the genus Ruoffia for which the name Ruoffia halotolerans sp. nov. is proposed. The type strain is INB8^T (= LMG 30291^T = CCUG 70701^T = QCC/B60/17^T).

从在卡塔尔内海收集的高盐度海水样品中分离出一种革兰氏染色阳性、无色素、球菌状、兼性厌氧和α-溶血细菌，命名为 INB8 ^T。该分离物能够在 25–40 °C（最佳，30 °C）、pH 5–11 和 2–8% NaCl 条件下生长。基于16S rRNA基因序列的系统进化分析表明菌株INB8 ^Ť放置在家庭内Aerococcaceae具有最高的序列相似性Facklamia tabacinasalis CCUG 30090 ^Ť（99.5％），其次是Facklamia人型支原体CCUG 36813 ^Ť（93.9％），Facklamia sourekii Y17312 ^T (93.8%),Facklamia ignava CCUG 37419 ^Ť（93.6％），Facklamia miroungae CCUG 42728 ^Ť（93.5％），Suicoccus acidiformans ZY16052 ^Ť（93.5％），Facklamia languida CCUG 37842 ^Ť（93.2％），Ignavigranum ruoffiae（93.1％），和Dolosicoccus paucivorans DSM 15742^吨（90.8%）。确定菌株 INB8 ^T和F. tabacinasalis CCUG 30090 ^T之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 94.5% 和 58.9%，证实了菌株 INB8 ^T代表一个新物种。主要脂肪酸是C _14:0、 C _16:0、 C _18:0和C _18:1 ω9c _。从基因组确定的菌株 INB8 ^T的 G + C 含量为 36.3 mol%。基于系统发育、化学分类学和表型信息，建议将Facklamia tabacinasalis重新分类为Ruoffia tabacinasalis，Facklamia ignava重新分类为Falseniella ignava，Facklamia sourekii重新分类为Hutsoniella sourekii。进一步提出菌株 INB8 ^T应归类为Ruoffia属的一个物种，其名称为Ruoffia halotolerans sp。十一月 被提议。类型菌株是 INB8 ^T (= LMG 30291 ^T  = CCUG 70701 ^T  = QCC/B60/17 ^T )。