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Reclassification of Facklamia ignava, Facklamia sourekii and Facklamia tabacinasalis as Falseniella ignava gen. nov., comb. nov., Hutsoniella sourekii gen. nov., comb. nov., and Ruoffia tabacinasalis gen. nov., comb. nov., and description of Ruoffia halotolerans sp. nov., isolated from hypersaline Inland Sea of Qatar

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Abstract

A Gram-stain-positive, non-pigmented, coccus-shaped, facultatively anaerobic and α-hemolytic bacterium designated as INB8T was isolated from a hypersaline marine water sample collected at the Inland Sea of Qatar. The isolate was able to grow at 25–40 °C (optimum, 30 °C), at pH 5–11 and with 2–8% NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain INB8T was placed within the family Aerococcaceae with the highest sequence similarity to Facklamia tabacinasalis CCUG 30090T (99.5%), followed by Facklamia hominis CCUG 36813T (93.9%), Facklamia sourekii Y17312T (93.8%), Facklamia ignava CCUG 37419T (93.6%), Facklamia miroungae CCUG 42728T (93.5%), Suicoccus acidiformans ZY16052T (93.5%), Facklamia languida CCUG 37842T (93.2%), Ignavigranum ruoffiae (93.1%), and Dolosicoccus paucivorans DSM 15742T (90.8%). Average nucleotide identity and digital DNA–DNA hybridization values between strain INB8T and F. tabacinasalis CCUG 30090T were determined to be 94.5% and 58.9% respectively, confirming strain INB8T represents a novel species. The major fatty acids were C14:0, C16:0, C18:0 and C18:1 ω9c. The G + C content of strain INB8T determined from the genome was 36.3 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic information, it is proposed that Facklamia tabacinasalis should be reclassified as Ruoffia tabacinasalis, Facklamia ignava be reclassified as Falseniella ignava, and Facklamia sourekii be reclassified Hutsoniella sourekii. It is further proposed that strain INB8T should be classified as a species of the genus Ruoffia for which the name Ruoffia halotolerans sp. nov. is proposed. The type strain is INB8T (= LMG 30291T = CCUG 70701T = QCC/B60/17T).

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Data availability

The 16S rRNA gene and genome sequences of strain INB8T have been deposited in GenBank under accession numbers KU747974 and JACAOA000000000, respectively.

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Acknowledgements

This work was supported by a research grant (NPRP 6-647-1-127) from the Qatar National Research Fund (a member of Qatar Foundation) to Rashmi Fotedar, We thank Aharon Oren (The Hebrew University of Jerusalem, Israel) for advice on naming the organism.

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Author notes

  1. Rashmi Fotedar and Paul A. Lawson have contributed equally to this work.

Authors and Affiliations

  1. Department of Genetic Engineering, Biotechnology Centre, Ministry of Municipality and Environment, Doha, Qatar

    Rashmi Fotedar, Aisha Zeyara, Amina Al Malki, Hamad Al Shamari & Masoud Al Marri

  2. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, 73019, USA

    Krithivasan Sankaranarayanan, Matthew E. Caldwell & Paul A. Lawson

  3. Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, OK, 73019, USA

    Krithivasan Sankaranarayanan

  4. Weill Cornell Medical College in Qatar, Doha, Qatar

    Ridhima Kaul

  5. Equine Veterinary Medical Centre, A Member of Qatar Foundation, Al Rayan, Qatar

    Mohammad Ali

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  1. Rashmi Fotedar
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Contributions

RF and PL designed research and project outline. RF, AZ, AM, RK, MM, HM performed collection, isolation, phenotypic and biochemical characterisation and 16S rRNA gene sequencing. MC performed the chemotaxonomic characterisation and repeat sequencing of 16S rRNA gene and KS performed the genome analysis. RF, PL and KS drafted the MS. All authors have read and approved the final manuscript.

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Correspondence to Rashmi Fotedar.

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10482_2021_1587_MOESM1_ESM.eps

Maximum-likelihood tree phylogenetic tree based on MurE gene demonstrating the relationship between INB8T and close relatives (EPS 2543 kb)

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Fotedar, R., Sankaranarayanan, K., Caldwell, M.E. et al. Reclassification of Facklamia ignava, Facklamia sourekii and Facklamia tabacinasalis as Falseniella ignava gen. nov., comb. nov., Hutsoniella sourekii gen. nov., comb. nov., and Ruoffia tabacinasalis gen. nov., comb. nov., and description of Ruoffia halotolerans sp. nov., isolated from hypersaline Inland Sea of Qatar. Antonie van Leeuwenhoek 114, 1181–1193 (2021). https://doi.org/10.1007/s10482-021-01587-7

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