Tweety-homolog 1 protein (Ttyh1) is abundantly expressed in neurons in the healthy brain, and its expression is induced under pathological conditions. In hippocampal neurons in vitro, Ttyh1 was implicated in the regulation of primary neuron morphology. However, the mechanisms that underlie transcriptional regulation of the Ttyh1 gene in neurons remain elusive. The present study sought to identify the promoter of the Ttyh1 gene and functionally characterize cis-regulatory elements that are potentially involved in the transcriptional regulation of Ttyh1 expression in rat dissociated hippocampal neurons in vitro. We cloned a 592 bp rat Ttyh1 promoter sequence and designed deletion constructs of the transcription factors specificity protein 1 (Sp1), E2F transcription factor 3 (E2f3), and achaete-scute homolog 1 (Ascl1) that were fused upstream of a luciferase reporter gene in pGL4.10[luc2]. The luciferase reporter gene assay showed the possible involvement of Ascl1, Sp1, and responsive cis-regulatory elements in Ttyh1 expression. These findings provide novel information about Ttyh1 gene regulation in neurons.

Tweety 同源物 1蛋白 ( Ttyh1 ) 在健康大脑的神经元中大量表达，并且其表达在病理条件下被诱导。在体外海马神经元中，Ttyh1 参与初级神经元形态的调节。然而，神经元中Ttyh1基因转录调控的机制仍然难以捉摸。本研究试图鉴定Ttyh1基因的启动子，并对可能参与体外大鼠离体海马神经元Ttyh1表达转录调控的顺式调控元件进行功能表征。我们克隆了 592 bp 的大鼠Ttyh1启动子序列，并设计了转录因子特异性蛋白 1 (Sp1)、E2F 转录因子 3 (E2f3) 和 achaete-scute 同源物 1 (Ascl1) 的缺失构建体，这些构建体融合在荧光素酶报告基因的上游在pGL4.10[ luc2 ]中。荧光素酶报告基因检测显示 Ascl1、Sp1 和响应性顺式调节元件可能参与Ttyh1表达。这些发现提供了有关神经元中Ttyh1基因调控的新信息。