Collection of skin very top layer, called stratum corneum, by tape stripping and the analysis of stratum corneum components by mass spectrometry provides multiple advantages for clinical studies that aim to understand the origins of allergic skin diseases and food allergy. However, such a methodology has multiple challenges on the way of complex stratum corneum analysis when molecules of different polarity are needed to be analyzed from minimal amount of skin tape strips. This review provides an overview of current knowledge about lipid and polar molecules in the skin, discusses challenging aspects of sample processing when dealing with skin tape strips, and provides some guidance towards approaches that generate complex, quantitative, normalized to total sample protein data that fit best the purpose of analysis of stratum corneum components for the purpose of clinical trials.

通过胶带剥离收集皮肤最表层（称为角质层）并通过质谱分析角质层成分，为旨在了解过敏性皮肤病和食物过敏起源的临床研究提供了多种优势。然而，当需要从最少量的皮肤胶带条中分析不同极性的分子时，这种方法在复杂的角质层分析方面存在多重挑战。本综述概述了有关皮肤中脂质和极性分子的当前知识，讨论了处理皮肤胶带时样品处理的挑战性方面，并为生成适合的复杂、定量、归一化总样品蛋白质数据的方法提供了一些指导最好是出于临床试验的目的分析角质层成分。