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Methodological Considerations for Lipid and Polar Component Analyses in Human Skin Stratum Corneum

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Abstract

Collection of skin very top layer, called stratum corneum, by tape stripping and the analysis of stratum corneum components by mass spectrometry provides multiple advantages for clinical studies that aim to understand the origins of allergic skin diseases and food allergy. However, such a methodology has multiple challenges on the way of complex stratum corneum analysis when molecules of different polarity are needed to be analyzed from minimal amount of skin tape strips. This review provides an overview of current knowledge about lipid and polar molecules in the skin, discusses challenging aspects of sample processing when dealing with skin tape strips, and provides some guidance towards approaches that generate complex, quantitative, normalized to total sample protein data that fit best the purpose of analysis of stratum corneum components for the purpose of clinical trials.

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Acknowledgements

This work was supported in part by NIH/NIAID Atopic Dermatitis Research Network (ADRN) Clinical Research Center grant 1U01AI152037.

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  1. National Jewish Health, 1400 Jackson Street, Goodman Building, K924b, Denver, CO, 80206, USA

    E. Berdyshev, I. Bronova, D. Y. M. Leung & E. Goleva

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Berdyshev, E., Bronova, I., Leung, D.Y.M. et al. Methodological Considerations for Lipid and Polar Component Analyses in Human Skin Stratum Corneum. Cell Biochem Biophys 79, 659–668 (2021). https://doi.org/10.1007/s12013-021-01016-6

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