Journal of Fluorescence ( IF 2.6 ) Pub Date : 2021-07-15 , DOI: 10.1007/s10895-021-02769-2 Annika Durve Gupta 1 , Arvind Gupta 2 , Almendra Reyes-Calderón 3 , Victor Ishrayelu Merupo 4 , Golap Kalita 5 , Jośe Herrera-Celis 6 , Naresh Chandra 1 , Ashutosh Sharma 3 , Jose Tapia Ramirez 7 , L G Arriaga 6 , Goldie Oza 6
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Metal sulfide nanoparticles are semi-conductors that possess many applications in optics, optoelectronics and magnetic devices. There are physical and chemical methods for their synthesis but such methods involve toxic precursors as well as many obnoxious by-products. Hence, biological synthesis of metal sulfide nanoparticles are efficient enough to transform toxic metals to non-toxic ones. Pseudomonas aeruginosa, isolated from textile effluent and tolerant of high levels of heavy metals, was used for the green synthesis of metal sulfide (HgS, As3S4, CdS and PbS) nanoparticles. The optical, structural and morphological nature of metal sulfide nanoparticles was also determined. FTIR (Fourier Transform Infra-red) analysis showed spectral changes when P. aeruginosa was grown in medium containing heavy metals viz. Hg, As, Pb and Cd indicating that there are functional groups viz. carboxyl, hydroxyl, phosphate, amino and amide, that exists on the surface of the bacteria, thus facilitating binding of metals on its surface. The bacterial samples which were treated with different metals at different concentrations, were subjected to whole cell protein analysis using SDS-PAGE (Sodium dodecyl Sulphate- Polyacrylamide gel electrophoresis) and protein profiling. The total protein estimation revealed that there was an increase in the protein concentration in the presence of heavy metals and a significant change in the banding pattern was observed which showed induction of a set of proteins under heavy metal stress especially mercury.
Graphical Abstract
中文翻译：
使用铜绿假单胞菌生物合成 PbS、As3S4、HgS、CdS 纳米颗粒及其结构、形态、光致发光以及全细胞蛋白质分析研究
金属硫化物纳米颗粒是一种半导体，在光学、光电子学和磁性器件中具有广泛的应用。它们的合成有物理和化学方法，但这些方法涉及有毒前体以及许多令人讨厌的副产物。因此，金属硫化物纳米颗粒的生物合成足以有效地将有毒金属转化为无毒金属。从纺织废水中分离出来的铜绿假单胞菌能够耐受高浓度的重金属，可用于金属硫化物（HgS、As 3 S 4 、CdS 和 PbS）纳米颗粒的绿色合成。还测定了金属硫化物纳米粒子的光学、结构和形态性质。 FTIR（傅里叶变换红外）分析显示，当铜绿假单胞菌在含有重金属的培养基中生长时，光谱会发生变化。 Hg、As、Pb 和 Cd 表明存在官能团，即。细菌表面存在羧基、羟基、磷酸基、氨基和酰胺基，从而有利于其表面金属的结合。使用不同浓度的不同金属处理的细菌样品，使用 SDS-PAGE（十二烷基硫酸钠-聚丙烯酰胺凝胶电泳）进行全细胞蛋白质分析和蛋白质分析。总蛋白质估计表明，重金属存在下蛋白质浓度增加，并且观察到条带图案的显着变化，这表明在重金属胁迫尤其是汞胁迫下诱导了一组蛋白质。
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