Background

Gold nanoparticles (AuNPs) have potential for a wide range of applications as therapeutic and diagnostic agents. Since they have a high probability of interacting with human immune cells, cytotoxicity studies must be conducted. The investigation of AuNP/immune cell interaction has mainly focused on macrophages and dendritic cells, along with some other cell lineages. Scarce information is available regarding the effect of AuNPs on mast cells, which are abundant in the skin, mucosa, and perivascular space.

Objective

To examine the uptake of AuNPs by HMC-1 human mast cells and the resulting effect on cell viability, pro-inflammatory mediators production, and proliferation.

Results

With AuNPs treatment, the viability of HMC-1 cells decreased slightly (never less than 95%) during the first 4 h, but no changes were detected in the proliferation rate at any time. Increasing concentrations of AuNPs produced greater cell granularity (uptake). CLSM images exhibited AuNPs clusters in the cell cytoplasm. TNF-α and ROS production was not stimulated by AuNPs treatment at any concentration/time.

Conclusion

Internalization of AuNPs into HMC-1 cells was demonstrated in an in vitro model, without showing cytotoxic effects or induction of pro-inflammatory mediators at any concentration tested.

中文翻译：

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金纳米粒子：人体肥大细胞的摄取和对细胞活力、炎症介质和增殖的影响

背景

金纳米粒子 (AuNPs) 具有作为治疗和诊断剂的广泛应用的潜力。由于它们与人体免疫细胞相互作用的可能性很高，因此必须进行细胞毒性研究。AuNP/免疫细胞相互作用的研究主要集中在巨噬细胞和树突细胞以及其他一些细胞谱系上。关于 AuNPs 对肥大细胞的影响的信息很少，肥大细胞在皮肤、粘膜和血管周围空间中很丰富。

客观的

研究 HMC-1 人类肥大细胞对 AuNP 的吸收以及由此产生的对细胞活力、促炎介质产生和增殖的影响。

结果

用 AuNPs 处理后，HMC-1 细胞的活力在前 4 小时内略有下降（从不低于 95%），但在任何时候都没有检测到增殖率的变化。增加 AuNP 的浓度会产生更大的细胞粒度（摄取）。CLSM 图像显示细胞质中的 AuNPs 簇。任何浓度/时间的 AuNPs 处理都不会刺激 TNF-α 和 ROS 的产生。

结论

在体外模型中证明了 AuNPs 内化到 HMC-1 细胞中，在任何测试浓度下都没有显示出细胞毒性作用或促炎介质的诱导。