Most of the 26 viruses that infect globe artichoke are detrimental to the crop’s production, quality, and nutritional value, hamper the development of a nursery activity in the respect of current EU legislation and pose risks for the new plantings. Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). The polyprobe described herein represent the first cheap and reliable example of a technology for broad-spectrum surveys in globe artichoke.

感染朝鲜蓟的 26 种病毒中的大多数都对作物的生产、质量和营养价值有害，阻碍了符合欧盟现行法规的苗圃活动的发展，并对新种植构成了风险。病毒控制的植物繁殖材料的全球移动需要快速、准确和可行的方法，以确保在一次检测中获得可靠的结果和检测多种病毒，以减少常规检测的时间和成本。在这里，我们描述了使用地高辛标记的多聚探针，该探针由合成的 50 nt 单链 DNA 序列组装而成，这些序列来自朝鲜蓟中最常见的病毒基因组。这些包括朝鲜蓟意大利潜伏病毒 (AILV)、朝鲜蓟潜伏病毒 (ArLV)、朝鲜蓟斑纹皱纹病毒 (AMCV)、萝卜花叶病毒 (TuMV)、番茄传染性萎黄病毒（TICV）、豆黄花叶病毒（BYMV）、黄瓜花叶病毒（CMV）、天竺葵带状斑病毒（PZSV）、番茄斑萎病毒（TSWV）和烟草花叶病毒（TMV）。此处描述的 polyprobe 代表了朝鲜蓟广谱调查技术的第一个廉价且可靠的示例。