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Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe

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Abstract

Most of the 26 viruses that infect globe artichoke are detrimental to the crop’s production, quality, and nutritional value, hamper the development of a nursery activity in the respect of current EU legislation and pose risks for the new plantings. Worldwide movement of virus-controlled plant propagative material require fast, accurate and feasible methods to guarantee reliable results and detection of multiple viruses in a single assay to reduce time and costs in routine tests. Here we describe the use of a digoxigenin-labelled polyprobe assembled from synthetic 50 nt single-stranded DNA sequences derived from genomes of the most commonly occurring viruses in globe artichoke. These included artichoke Italian latent virus (AILV), artichoke latent virus (ArLV), artichoke mottled crinkle virus (AMCV), turnip mosaic virus (TuMV), tomato infectious chlorosis virus (TICV), bean yellow mosaic virus (BYMV), cucumber mosaic virus (CMV), pelargonium zonate spot virus (PZSV), tomato spotted wilt virus (TSWV) and tobacco mosaic virus (TMV). The polyprobe described herein represent the first cheap and reliable example of a technology for broad-spectrum surveys in globe artichoke.

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Acknowledgements

This work was prevalently granted by Agritest S.r.L., Valenzano (BA), southern Italy, to apply for the European patent EP 2 982 763 “Synthesis and use of a polyprobe for the detection of viral infections in globe artichoke”. Validation tests were carried out in the framework of the project “Resources with low environmental impact and innovative cultivation systems in the production processes of the regional supply chain of the early flowering globe artichoke (RICAR)” grant PRS 116 by the Apulian Regional Government (southern Italy).

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  1. Serena Anna Minutillo

    Present address: Mediterranean Agronomic Institute of Bari (CIHEAM-IAMB), Via Ceglie, 9 BA, 70010, Valenzano, Italy

Authors and Affiliations

  1. Dipartimento di Scienze del Suolo della Pianta e Degli Alimenti, Università degli Studi di Bari Aldo Moro, Via Amendola 165/A, 70126, Bari, Italy

    Serena Anna Minutillo, Roberta Spanò, Donato Gallitelli & Tiziana Mascia

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  1. Serena Anna Minutillo
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  2. Roberta Spanò
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  3. Donato Gallitelli
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  4. Tiziana Mascia
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Correspondence to Tiziana Mascia.

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Minutillo, S.A., Spanò, R., Gallitelli, D. et al. Simultaneous detection of 10 viruses in globe artichoke by a synthetic oligonucleotide-based DNA polyprobe. Eur J Plant Pathol 160, 991–997 (2021). https://doi.org/10.1007/s10658-021-02292-x

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  • DOI: https://doi.org/10.1007/s10658-021-02292-x

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